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...s added to the [[N-pathway control state]] in a defined [[coupling-control state]]. |info=[[Flux control efficiency]], [[Gnaiger 2020 BEC MitoPathways]]

...erentially as reference state, if mitochondria are studied with respect to N-pathway injuries. ...~ GM''_P''). Low N/S flux control ratios indicate defects in the N-pathway upstream of the Q-junction.

...TS competent N-linked substrate combinations) and the [[NS-pathway control state]] (N in combination with succinate). NS indicates respiration with a cockta |mitopedia concept=Respiratory control ratio

...t|100px|link=http://www.bioblast.at/index.php/O2k-Publications: Topics|O2k-Publications: Topics]] [[File:SUIT-catg FNSGp.jpg|right|200px|link=Electron-transfer-pathway state|Electron-transfer-pathway states]]

..., S(Rot) or S. Then the NS-S pathway control efficiency in the ET-coupling state is ...to the NS-state. The reversed protocol, adding N-substrates to a S-pathway control background does not provide a valid estimation of S-respiration with succin

...ing N-type substrates to a [[S-pathway control state]] as the [[background state]] does not provide a valid estimation of S-linked respiration with succinat = List of publications: S and NS pathway control states =

...alonic acid]]). In the N-pathway control state [[Electron-transfer-pathway state|ET pathway level 4]] is active. |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]

...y_state|N]], [[NS-pathway control state| NS]], [[Succinate pathway control state| S]]). SUIT-004 can be extended with the CIV assay module. ...n| ''P'']]-[[ET capacity| ''E'']]) with N substrates ([[PM-pathway control state|PM]]).

|abbr= [[Malate-anaplerotic pathway control state|Malate anaplerosis]] |info='''A:''' '''[[Malate-anaplerotic pathway control state|Malate anaplerotic pathway]]'''

...rnatively to a '''Complex I-linked substrate state''' ([[N-pathway control state]]). == List of publications: Complex I ==

|description=[[File:SUIT-catg NS.jpg|right|300px|NS-pathway control]] ...pt in terms of convergent electron transfer to the [[Q-junction]] (pathway control). '''NS''' is the abbreviation for the combination of [[NADH]]-linked subst

...ion_pathway_control_state|F]], F(N), [[FN]] and [[FNS]] pathways in OXPHOS state can be evaluated by using this SUIT protocol. ...n of malate used in this protocol, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway.

...ed pathway control) in combination with [[succinate]] ([[S-pathway control state]]; S- or CII-linked) and one or several fatty acids, which are supplied to |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]

...n of malate used in this protocol, typically 0.1 mM, does not saturate the N-pathway; but saturates the F-pathway. :::+ F-pathway (3Oct-2M.1) can be compared to FN-pathway (5P) in OXPHOS state.

|abbr=[[Glutamate-anaplerotic pathway control state|Glutamate anaplerosis]] |info='''A:''' '''[[Glutamate-anaplerotic pathway control state|Glutamate anaplerotic pathway]]'''

...r through the F-junction (which is typically rate-limiting relative to the N-pathway branch), but simultaneously generates FADH₂ and NADH and thus de |info=[[Electron-transfer-pathway state]], [[Gnaiger 2020 BEC MitoPathways]]

...are designed to measure simultaneously O₂ flux and the Q-redox state in isolated mitochondria and permeabilized cells. ...t D072]] simultaneous determination of O₂ flux and the Q-redox state in isolated mitochondria and cells permeabilized previously to addition to

|info='''A''' Linear coupling control with NADH-linked substrates for isolated mitochondria, tissue homogenate an ...without the involvement of the [[complex II]] ([[Succinate pathway control state| S-pathway]]). It can be useful to understand the contribution and the acti

...rylation|OXPHOS state]], and [[FN]]—[[FNS]]—[[S]] in the [[ET capacity| ET state]]. ...in the fatty acid oxidation (F) and fatty acid oxidation&NADH pathway (FN) control states.

...OS]] with [[NS-pathway control state|NS substrates]]) and coupling/pathway control''' ...ET state ([[NS-pathway control state|NS]] and [[Succinate pathway control state|S]]). SUIT-011 can be extended with the CIV assay module.

...ce of ATPases in [[mitochondrial preparations]] and is useful as a quality control of the sample preparation. ...combinations for N-pathway control state; S or RotS for S-pathway control state.

...-[[ET capacity| ''E'']]) with NADH-linked substrates ([[PM-pathway control state|PM]] and PGM)''' ...may be relevant in the presence of [[glutamate-anaplerotic pathway control state]]. SUIT-012 can be extended with the CIV assay module.

...otic pathway, and therefore could be used to study [[F-pathway]] without [[N-pathway]] contribution in samples with mt malic enzyme. :::- This protocol does not analyze [[Coupling control state|coupling control]].

...GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI ([[Lemieux 2017 Sci Rep|Lemieux ''et al.'' 2017]]; [[Votion ...say for the diagnosis of injuries in the N-pathway, since an impairment of N-pathway capacity can be compensated partially by activation of the S-pathway. This

|info='''A''' to analyse O₂ flux in the NS- pathway control state on isolated mitochondria- '''[[SUIT-031]]''' ...006 O2 mt D022]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols

|info='''A''' to analyse O₂ flux in the NS- pathway control state in permeabilized cells- '''[[SUIT-031]]''' ...006 O2 mt D022]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols

! Respiratory state ! Pathway control

...alyse the coupling control on permeabilized cells in N(PM) pathway control state- '''[[SUIT-006]]''' :::* This protocol is indicated when the aim is to analyse coupling control in permeabilized cells in NADH-pathway using PM as substrates.

::: '''[[SUITbrowser]]:''' [[Glutamate-anaplerotic pathway control state|Glutamine/glutamate]] ...lutamate alone allows to analyze the glutamate-anaplerotic pathway control state.

|info='''A: quality control evaluation of [[mitochondrial preparations]]''' - '''[[SUIT-029]]''' ...diagnosis of injuries in the N-pathway, since in the case of GM impairment N-pathway capacity can be compensated partially by activation of the S-pathway.

...and ET state ([[NS-pathway control state|NS]], [[Succinate pathway control state|S]]) is also evaluated, covering the contribution of two pathways which are ...b>''P''</sub>. SUIT-004 should be chosen for the additive effect in the ET-state.

|info='''A: Additivity between the N- and [[Succinate pathway control state| S-pathway]] in the Q-junction''' ...NADH_Electron_transfer-pathway_state| N-]] and [[Succinate pathway control state| S-pathway]] in the [[Q-junction]], providing a physiologically relevant es

...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...of O₂ flux on isolated mitochondria and tissue homogenate as a control for SUIT-020 Fluo mt D033.

...support NADH-linked respiration. Oligomycin (Omy) is used to induce a LEAK state of respiration via the inhibition of the ATP synthase. Since higher concent ...of O₂ flux on isolated mitochondria and tissue homogenate as a control for SUIT-021 Fluo mt D036.

...ency |''P-L'' control efficiency]] if ''P=E'' as evaluated in a [[coupling-control protocol]]. |info=[[Flux control efficiency]]

...ity| ''E'']]) with NADPH-linked substrate substrates ([[PM-pathway control state|PM]]). ...y_state|N]], [[NS-pathway control state| NS]], [[Succinate pathway control state| S]]).

...f the ATP synthase. Since higher concentrations of Omy can decrease the ET state induced upon addition of uncoupler, the required concentration of Omy has t ...GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI.

...hich leads to an overestimation of LEAK respiration, when comparing [[LEAK state without adenylates|''L''(n)]] and [[LEAK respiration|''L''(Omy)]]. Tissue h ...in [[mitochondrial preparations]]. It is particularly useful as a quality control of the purity of isolated mitochondria.

...ith [[SUIT-001]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols ...y test]] for inter-individual and inter-laboratory reproducibility quality control.

...e consisting of three coupling control states. The term '''phosphorylation control protocol''', PCP, has been introduced synonymous for CCP. ...sted to replace the term 'phosphorylation control protocol' by '''coupling-control protocol, CCP'''.

...ays) and in [[ET capacity| ET state]] ([[FNSGp]] and [[SGp-pathway control state|SGp]]) can be evaluated by using this SUIT protocol. SUIT-002 can be extend ...ith [[SUIT-001]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols

...GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI. ...sed [[Mitochondrial membrane potential|mt-membrane potential]] in the LEAK state.

...espiratory capacities, N + S, is identical to the capacity measured in the state with combined substrates, NS (CI<small>&</small>II). This condition of comp = List of publications: N and S and NS (CI and CII and CI<small>&</small>II) =

...analyse O2 flux and the Q-redox state in the N-,NS- and S- pathway control state on isolated mitochondria- '''[[SUIT-031]]''' ...-006 Q mt D071]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols

...[[FNS]], [[Succinate pathway control state| S]] and [[SGp-pathway control state| SGp]] pathways) can be evaluated by using this SUIT protocol. SUIT-001 can ...ith [[SUIT-002]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols

...grity can be evaluated by the addition of cytochrome ''c'' ([[Cytochrome c control factor |cytochrome ''c'' test]]). The early addition of cytochrome ''c'' i ...assay for the diagnosis of injuries in the N-pathway, since impairment of N-pathway capacity can be compensated partially by activation of the S-pathway. This

...osphorylation|OXPHOS state]] and of [[FNS]] and [[S]] in [[ET capacity| ET state]] is evaluated. ...GM- with PM-capacity yields important information on N-pathway respiratory control upstream of CI ([[Lemieux 2017 Sci Rep|Lemieux ''et al.'' 2017]]; [[Votion

...'A''' to analyse O2 flux and the Q-redox state in the NS- pathway control state on permeabilized cells- '''[[SUIT-031]]''' ...Q ce-pce D073]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols

...tocol and Crabtree effect test -'''[[SUIT-003]]''' complex cell [[coupling-control protocol]] :::* The SUIT-003 O2 ce D038 is a cell coupling-control protocol. The total cell count (''N''_ce) is the sum of viable ce

...assay for the diagnosis of injuries in the N-pathway, since impairment of N-pathway capacity can be compensated partially by activation of the S-pathway. This ...with NP + SP. SUIT-004 should be chosen for the additive effect in the ET-state.

...ration#The_LEAK_state |LEAK state]] and [[Oxidative phosphorylation|OXPHOS state]]''' ...] in [[LEAK respiration#The_LEAK_state |LEAK state]] and [[ET capacity| ET state]] is evaluated.

...ith [[SUIT-001]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols ...y test]] for inter-individual and inter-laboratory reproducibility quality control.

|info='''A''' - '''[[SUIT-006]]''' - Protocol for coupling control assessment in mitochondrial preparations (isolated mitochondria, tissue hom ...''see'' [[MitoPedia: Sample preparations]]) at [[Succinate pathway control state]].

...rations of [[ADP]] and inorganic phosphate (which may not be the case in [[State 3]]), oxygen, and defined reduced CHNO-fuel substrates. ...possible in mt-preparations supported by an ET-pathway competent substrate state, exemplifed as the NS-pathway (CI<small>&</small>II-linked substrate supply

|info='''A''' '''[[SUIT-003]]''' - [[Coupling-control protocol]] and [[Crabtree effect]] :::* The SUIT-003 O2 ce D037 is a coupling-control protocol for living cells. The total cell count (''N''_ce) is the

...b> consumption and the redox state of the Q-pool in S(Rot)-pathway control state in isolated mitochondria- '''[[SUIT-006]]''' ...-031 Q mt D072]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols

...say for the diagnosis of injuries in the N-pathway, since an impairment of N-pathway capacity can be compensated partially by activation of the S-pathway. This ...with NP + SP. SUIT-004 should be chosen for the additive effect in the ET-state.

|abbr=[[Coupling-control protocol |CCP-mtprep]] |info='''A: [[Coupling-control protocol]], [[Mitochondrial preparations |mtprep]]'''

...ith [[SUIT-001]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols ...y test]] for inter-individual and inter-laboratory reproducibility quality control.

...ith [[SUIT-001]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols ...y test]] for inter-individual and inter-laboratory reproducibility quality control.

...OS capacity| OXPHOS capacity]] in the [[malate-anaplerotic pathway control state]]. ...OS capacity| OXPHOS capacity]] in the [[malate-anaplerotic pathway control state]].

...n the absence of other, potentially interfering pathways, both in the LEAK state and in OXPHOS. ...ays) and in [[ET capacity| ET state]] ([[FNSGp]] and [[SGp-pathway control state|SGp]]) can be evaluated by using this SUIT protocol.

...ith [[SUIT-002]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols ...y test]] for inter-individual and inter-laboratory reproducibility quality control.

...b> consumption and the redox state of the Q-pool in S(Rot)-pathway control state in permeabilized cells- '''[[SUIT-006]]''' ...Q ce-pce D074]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols

|abbr=''ET-pathway state'' ...state]]s. [[Coupling-control state]]s require [[Electron-transfer-pathway state|ET-pathway competent states]], including oxygen supply. [[Categories of SUI

...that supports succinate-linked respiration (succinate-induced respiratory state; previously used nomenclature: CII-linked respiration; SRot; see [[Gnaiger The S-pathway control state is usually induced in mt-preparations by addition of succinate&rotenone. In

...ity from mouse to man, and evaluation of altered mitochondrial respiratory control patterns in health and disease. :::::* Lemieux H, Blier PU, Gnaiger E (2017) Remodeling pathway control of mitochondrial respiratory capacity by temperature in mouse heart: electr

...ith [[SUIT-002]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols ...y test]] for inter-individual and inter-laboratory reproducibility quality control.

...in the LEAK state. Pyruvate (P) supports [[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usually in the presence of ADP it does not increase ...s protocol is designed to evaluate RET in the S-linked pathway in the LEAK state, which is decreased by ADP due to reduction of the mt-membrane potential.

...ith [[SUIT-002]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols ...est]] for inter-individual and inter-laboratory reproducibility as quality control.

.../sub> calibration steps]] are done during the experiment. The experimental control for O₂ flux in the absence AmR is carried out with [[SUIT-009 O2 ...pce D016]] determination of O₂ flux on permeabilized cells as a control for SUIT-009 AmR ce-pce D019 to test the inhibitory effect of the fluoresce

...rates: 1. Electron transfer pathways and respiratory control. 2. Coupling control. ...Strategically designed SUIT protocols reveal a diversity of mt-respiratory control patterns and pathway additivity depending on species, organs, cell types, a

...n in the LEAK state. Pyruvate (P) promotes[[NADH Electron transfer-pathway state|NADH-pathway (N)]], and usually in the presence of ADP, H₂O<sub ...ized cells (already permeabilized when they are added to the chamber) as a control for SUIT-009 AmR mt D021

...ity from mouse to man, and evaluation of altered mitochondrial respiratory control patterns in health and disease'' ([[Gnaiger 2009 Int J Biochem Cell Biol|Gn ...dered as keywords which can be attached to various sources of information (publications). With proper labelling, multidimensional maps of information can be constr

...Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://d ! Name !! State !! Abbreviation !! Example

[[Image:O2k-Publications.jpg|right|200px|link=O2k-Publications|O2k-Publications: chronological]] [[Image:MiPMap Publication.jpg|right|200px|link=MiPMap|Publications in the MiPMap]]

...ith [[SUIT-002]] provides a common reference for comparison of respiratory control in a large variety of species, tissues and cell types. Both SUIT protocols ...y test]] for inter-individual and inter-laboratory reproducibility quality control.

...xaloacetate on the succinate dehydrogenase. This protocol can be used as a control to investigate the effect of the fluorescence dyes on the respiration. :::+ Cytochrome ''c'' test can be performed in the S-OXPHOS state.

...easurement and expression of respiration vary ([[oxygen flux]] in the LEAK state, ''J''_O₂''L'', or [[oxygen flow]], ''I''<sub>O<sub>2< ...stem and in mt-preparations supported by an ET-pathway competent substrate state, exemplifed as the NS-pathway (CI<small>&</small>II-linked substrate supply

...upling-control protocol]] (CCP) in living cells (ce) with oligomycin (LEAK state) :::* The CCP-ce (SUIT-003 O2 ce D009) is a [[coupling-control protocol]] for living cells. In a living cell population, the total cell co

...(succinate&rotenone) restricts ET-capacity and artificially enhances flux control upstream of the Q-cycle, providing diagnostic information on specific branc ...e experimental basis for high accuracy of quantitative results and quality control in high-resolution respirometry.

...iling of snap frozen muscle biopsies was performed by LC/MS/MS at Colorado State University. ...XPHOS measures correlated as strongly as their relative expression as flux control factors, which paralleled greater expression of mitochondrial phosphate car

::: '''Concept of joint publications on nomenclature and terminology in mitochondrial physiology''' ...ry states' to include [[MitoPedia: Respiratory control ratios |respiratory control ratios]].

...rence between ''E'' and ''P''. In many types of mitochondria, the [[OXPHOS-control ratio|''P/E'' ratio]] is >1.0 [1,2]. ...tion to OXPHOS Analysis. Mitochondr Physiol Network 17.18. Oroboros MiPNet Publications, Innsbruck: 64 pp.]]

::: '''[[SUIT protocol pattern]]:''' bidirectional linear [[coupling-control protocol]] 1PM;2D;3Omy;4U;5Ama ...h fluorescent dyes. In this protocol, the [[NADH Electron transfer-pathway state]] can be analyzed in [[Mitochondrial preparations| mitochondrial preparatio

...f 16%, 26%, and 35% for the leak state, ADP-simulated state, and uncoupled state, respectively, relative to rates using B1. The common feature distinguishin ::::» [[O2k-brief |List of O2k-Publications presented as O2k-brief]]

...lso advisable to either detect H₂O₂ flux at the same state for high (tissue hyperoxia; [O₂]> ~100-110 µM) and low (tissue ...end of the protocol to study the effect of oxygen concentration in the ROX state (in the presence of Ama) both on the O₂ and H₂O<sub>2

...ent. It is advisable to detect H₂O₂ flux in the same state either at high oxygen concentration (tissue hyperoxia; [O₂]> ~10 ...x in isolated mitochondria, tissue homogenate and permeabilized cells as a control to test the effect of the fluorescence dye on the respiration.

|info='''A: [[Coupling-control protocol]], [[living cells]]''' »[http://wiki.oroboros.at/images/d/da/MiPN ...commended in the SUIT-003 have been designed to study the coupling control state of living cells. In order to study the [[LEAK respiration]], the phosphoryl

...b>''P''</sub>. SUIT-004 should be chosen for the additive effect in the ET-state. ...herefore, this ''Rox'' measurement is frequently taken as a methodological control rather than as the final basis of ''Rox'' correction of mitochondrial respi

...rformance (2) provide essential reference points for analysis of metabolic control mechanisms and diagnosis of mitochondrial function in health and disease. ...rformance (2) provide essential reference points for analysis of metabolic control mechanisms and diagnosis of mitochondrial function in health and disease.

Quality first: a paradigm shift in mt-respiratory control towards global connection. ...f 'papers') indicates that a paradigm shift is in action from conventional publications (all of which should be Open Access) to structured databases (all of which

...ry control and OXPHOS analysis, reflected in [[O2k-Publications|>1,000 O2k-publications]] using the Oroboros Oxygraph-2k. The [[O2k-Fluorescence LED2-Module]] is a ...tochondria]] was not only a function of respiratory substrate and coupling state, but depended strongly on experimental oxygen levels below and even above a

[[Image:O2k-Publications.jpg|left|80px|link=O2k-Publications: Topics|O2k-Publications in the MiPMap]] :::* Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. - [[Gn

...e experimental basis for high accuracy of quantitative results and quality control in high-resolution respirometry. ::::» [[O2k-brief |List of O2k-Publications presented as O2k-brief]]

...matic control by [[DatLab]] of programmable titration regimes and feedback control (oxystat, pH-stat). ...s injections for steady-state respirometry, (both operated from the direct control mode), and feedback controlled operation.

...indicating elevated electron and proton leak and only slightly elevated E state respiration in blood platelet mitochondria (p=0.0534). ....) Mitochondrial Pathways and Respiratory Control. 2nd ed. Oroboros MiPNet Publications, Innsbruck 62–73.

...TPases are present in the sample, it is not possible to reach the [[LEAK]] state. ...solated mitochondria. Similar protocol without uncoupler titrations and ET state evaluation.

...TPases are present in the sample, it is not possible to reach the [[LEAK]] state. ...n isolated mitochondria. Similar protocol with uncoupler titrations and ET state evaluation.

|title=On coupling control of mitochondrial respiration. What is wrong with the RCR? ...o zero in a fully coupled system. Maximal efficiency in the ADP-stimulated state of oxidative phosphorylation (OXPHOS) is, however, not adaptive [1] and is

...to be determined if used. Higher concentrations of Omy may inhibit the ET state. ...solated mitochondria. Similar protocol without uncoupler titrations and ET state evaluation.

...e in fatty acid oxidation approaching the level of the Inuit, yet coupling control of oxidative phosphorylation was conserved. Our findings reveal that couple |keywords=[[Flux control factor]]

...le=Gnaiger E, Steinlechner-Maran R, Méndez G, Eberl T, Margreiter R (1995) Control of mitochondrial and cellular respiration by oxygen. https://doi.org/10.100 ...tion is paid to the notion of complementary kinetic and thermodynamic flux control mechanisms.<br>

...[[SUIT-018 AmR mt D040]] simultaneously at high oxygen concentration as a control. ...ub>2</sub>O₂ flux on the mt-membrane potential on the N-control state in mitochondrial preparations.

...ub>2</sub>O₂ flux on the mt-membrane potential on the N-control state in mitochondrial preparations. {{#ask:[[Category:Publications]] [[Additional label::SUIT-018]] [[Instrument and method::O2k-Protocol]]

...ts as bioenergetic biomarkers of changes in brain mitochondria in diabetic state. ...jected with streptozocin (STZ) at the dose of 65 mg/kg of body weight. The control group of animals (healthy) received a vehicle (citrate buffer). Animals, in

|title=Cytochrome c flux control factor as a quality criterion in respiratory OXPHOS analysis in canine perm ...during isolation [2]. The cytochrome ''c'' effect is expressed as the flux control factor ''FCF_c'', which is the increase of OXPHOS capacity after

...e 3]] respiration (IC(50) = 192 ± 17 nM), with no significant effects on [[State 4]](o). An inhibition in the activity of both Complex I and V was also obse ...''L/E'' ratio (LEAK/ET capacity) would provide information on the coupling state [5,6].

Two exciting publications on '''mitochondrial [[p50|''p''₅₀]]''', apparent [[excess capaci ..., Iglesias-Gutierrez E, Zierath JR, Garcia-Roves PM (2012) Tissue-specific control of mitochondrial respiration in obesity-related insulin resistance and diab

::::# Instrumental quality control with DL-Protocols. | Padang State University (ID)

...sub> levels above the respiratory oxygen ''control'' region. In the oxygen control region, the capacity for O₂ consumption is compromised by hypoxi :::: The concept of updated '''versions''' of publications in BEC is promoted, to quickly add corrections and minor updates without re

...n isolated mitochondria. Similar protocol with uncoupler titrations and ET state evaluation. :::* [[SUIT-032 O2 mt D109]]: Control protocol for respiration only, allowing for cytochrome ''c'' test.

...naiger: Assessment of mitochondrial and cellular oxygen kinetics by steady-state oxygen titrations, using the Titration-Injection microPump TIP2k, '''Interplay of respiratory components and mitochondrial diaphorases on redox state in anoxia'''

...mitochondrial respiratory control and OXPHOS analysis, reflected in >1,400 publications using the Oroboros Oxygraph-2k. The O2k-Fluorometer is a recent extension o ...yeast cells was not only a function of respiratory substrate and coupling state, but depended strongly on experimental oxygen levels below and even above a

...2020) Commitment to reproducibility in targeting mitochondrial respiratory control: basic and advanced applications of the O2k-FluoRespirometer. PaduaMuscleDa ...itness and early aging. High-resolution respirometry (HRR) is based on the state-of-the-art instrument (Oroboros O2k, Innsbruck, Austria) for mitochondrial

...ngful interpretation. Reduction of the number of irreproducible zero-value publications is the most effective measure to reduce the paper mass excess (PME) in the [[File:PubMed count-mito.png|right|500px|thumb|'''Figure 6.1. Number of publications on mitochondria per year'''. Nobel price laureates are marked who are parti

...|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] ...|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]

...OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. Oroboros MiPNet Publications, Innsbruck: 64 pp. ...on oxidative phosphorylation in liver, brain, and heart mitochondria using State 3 oxidation rate assays. Toxicol Appl Pharmacol 249: 65-75.

== O2k-Publications == ...glycolytic ATP production a paradox? In: Surviving Hypoxia: Mechanisms of Control and Adaptation. Hochachka PW, Lutz PL, Sick T, Rosenthal M, Van den Thillar

::::Day 1: Mitochondrial respiratory capacity, coupling and respiratory control. Programme 1 - Abstracts 1 ...ing Lectures. Mitochondrial respiratory capacity, coupling and respiratory control - Abstracts Day 1

...present results on patients, but is aimed at a characterization of healthy control groups. Exclusion criteria in the pre-analytical phase are related to smoki :::::# Time of sampling, fasting and resting state require standardization. Anticoagulants are used for whole blood sampling (

...OXPHOS analysis. 3rd ed. Mitochondr Physiol Network 17.18. Oroboros MiPNet Publications, Innsbruck:64 pp. ISBN 978-3-9502399-6-6 (see 5th edition: [[Gnaiger 2020 B ...7) Mitochondrial Pathways and Respiratory Control. 1st ed. Oroboros MiPNet Publications, Innsbruck: 96 pp]]

...ysis. 4^th ed. Mitochondr Physiol Network 19.12. Oroboros MiPNet Publications, Innsbruck:80 pp. — <big>'''''see 5^th edition: [[Gnaiger 2020 ...tates |Respiratory states]], [[MitoPedia: Respiratory control ratios |Flux control ratios]]

...tics. We endeavour to provide a balanced view on mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in ...ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux, O₂

...activity of living (non-permeabilized) cells in the physiological coupling state, which is controlled by cellular energy demand, energy turnover and the deg |'''Why use a short-chain coenzyme Q mimetic for measurement of the Q-redox state using the Q-Module? '''<br>

...|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] ...|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]

:::::* Concept of joint publications on nomenclature and terminology in mitochondrial physiology. ...uscript on protocols for blood cell isolation and storage - summary of the state-of-the-art.

...2) Mitochondrial Pathways and Respiratory Control. 3rd ed, OROBOROS MiPNet Publications, Innsbruck''' *Cell Respiration and Phosphorylation Control: [[MiPNet08.09]]

|title=Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5^th ed. Bioenerg Commun 202 ...ance to society. The ‘Blue Book’ on Mitochondrial Pathways and Respiratory Control presents a fundamental introduction to OXPHOS analysis for students and res

...titration of AmR after a specific respiratory state has been reached in a control experiment. ...towards H₂O₂, which, therefore, serves as a quality control step. The sensitivity depends on the respiration medium and on the componen

...|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] ...|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]

...unction in rat heart, liver and skeletal muscle.” [[Cecatto_C#Publicatons|»Publications«]] ...inical routine antidepressant treatment. - [[Karabatsiakis_A#Publications|»Publications«]]

...|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]] ...|70px|link=http://wiki.oroboros.at/index.php/O2k-Publications:_Topics |O2k-Publications in the MiPMap]]

| Quality control assessment of mitochondrial respiration- ''completed'' ::::# Full C.V. (including a list of academic publications – if applicable).

...andard protocols will rigorously document (Maelstrom Research program) the state-of-the-art in designing, conducting, reporting, interpreting, and validatin ...'']] (ORO) '''Release of DatLab 7''' - new dimensions with MitoFit quality control and MitoPedia: DatLab.

...tics. We endeavour to provide a balanced view on mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in ...ET; proton leak, LEAK, residual oxygen consumption, ROX, State 2, State 3, State 4, normalization, flow, flux, O₂

...certed action of the scientific community is required to implement quality control systems and optimally harmonize protocols across research groups. The prima ...cedures will be compared and strategies defined for improvement of quality control. An inter-laboratory ring test will be established as a world-wide innovati

...sense, the Strophes turn back on quality ''versus'' quantity of scientific publications with reflections on publishing and ''bug''lishing in the spirit of Charles ...by the scientific establishment. Nevertheless, the majority of scientific publications are irreproducible waste (''see'' [[Publication efficiency]]), mainly as a

...tics. We endeavour to provide a balanced view of mitochondrial respiratory control and a critical discussion on reporting data of mitochondrial respiration in ...rol ratio]] • [[Electron transfer pathway]] • [[Flow]] • [[Flux]] • [[Flux control ratio]] • [[IUPAC]] • [[LEAK respiration]] • [[Mitochondrial marker]]