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O2k-Publications: Instruments;methods

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 Was published in yearHas title
MiPNet18.10 O2k-Specifications2024-03-26
Oroboros
O2k-specifications for respirometry and comprehensive OXPHOS analysis.
Tsouka 2024 Commun Med (Lond)2024Tsouka S, Kumar P, Seubnooch P, Freiburghaus K, St-Pierre M, Dufour JF, Masoodi M (2024) Transcriptomics-driven metabolic pathway analysis reveals similar alterations in lipid metabolism in mouse MASH model and human. Commun Med (Lond) 4:39. https://doi.org/10.1038/s43856-024-00465-3
MiPNet28.07 NextGen-O2k Series XB manual2023-10-19
O2k-Manual
NextGen-O2k Series XB manual
MiPNet28.08 Oroboros O2k Series J manual2023-10-19
O2k-Manual
Oroboros O2k Series J manual
MiPNet14.06 Instrumental O2 background2023-10-19
O2k-Manual
O2k Quality Control 2: Instrumental oxygen background correction and accuracy of oxygen flux.
MiPNet28.10 SmartPOS-service2023-09-29
O2k-Manual
SmartPOS: Service
MiPNet19.14 SOP Hamilton microsyringes2023-09-28
O2k-Manual
SOP for manual O2k-titrations with Hamilton microsyringes.
MiPNet28.09 O2k-Fluo Smart-Module manual2023-09-25
O2k-Manual
O2k-Fluo Smart-Module manual
MiPNet19.03 O2k-cleaning and ISS2023-03-14
O2k-Manual
O2k-Chamber cleaning SOP and Integrated Suction System (ISS).
MiPNet22.11 O2k-FluoRespirometer manual2023-03-14
O2k-Manual
O2k-FluoRespirometer manual.
MiPNet06.03 POS-calibration-SOP2023-02-06
O2k-Manual
O2k Quality Control 1: Polarographic oxygen sensors and accuracy of calibration.
Ebanks 2023 PLoS One2023Ebanks B, Kwiecinska P, Moisoi N, Chakrabarti L (2023) A method to assess the mitochondrial respiratory capacity of complexes I and II from frozen tissue using the Oroboros O2k-FluoRespirometer. https://doi.org/10.1371/journal.pone.0276147
Nord 2023 FASEB J2023Nord A, Chamkha I, Elmér E (2023) A whole blood approach improves speed and accuracy when measuring mitochondrial respiration in intact avian blood cells. https://doi.org/10.1096/fj.202201749r
Mu 2023 STAR Protoc2023Mu C, Shearer J (2023) Protocol for measuring respiratory function of mitochondria in frozen colon tissue from rats. https://doi.org/10.1016/j.xpro.2023.102560
Giovarelli 2023 Mol Med2023Giovarelli M, Serati A, Zecchini S, Guelfi F, Clementi E, MandĂČ C (2023) Cryopreserved placental biopsies maintain mitochondrial activity for high-resolution respirometry. https://doi.org/10.1186/s10020-023-00645-2
MiPNet26.14 Oxia2022-10-31
O2k-Manual
Oxia - HyperOxia to HypOxia.
MiPNet27.07 Sample Holder2022-08-09
O2k-Manual
Sample Holder.
MiPNet26.13 NextGen-O2k manual2022-07-25
O2k-Manual
NextGen-O2k manual
MiPNet26.12 NextGen-O2k: NADH-Module2022-05-17
O2k-Manual
NextGen-O2k: NADH-Module manual
Komlodi 2022 BEC2022Komlódi T, Tretter L (2022) The protonmotive force – not merely membrane potential. Bioenerg Commun 2022.16. https://doi.org/10.26124/bec:2022-0016
Baglivo 2022 MitoFit-QC2022Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. https://doi.org/10.26124/mitofit:2022-0018.v2 — 2022-08-04 published in Bioenerg Commun 2022.8.
Hansen 2022 Magn Reson Med2022Hansen K, Hansen ESS, Jespersen NRV, BĂžtker HE, Pedersen M, Wang T, Laustsen C (2022) Hyperpolarized 13C MRI reveals large changes in pyruvate metabolism during digestion in snakes. https://doi.org/10.1002/mrm.29239
Meza-Buendia 2022 PLoS One2022Meza-Buendia AK, Aparicio-Trejo OE, Díaz F, Caamal-Monsreal C, Pedraza-Chaverri J, Álvarez-Delgado C, Paschke K, Rosas C (2022) High resolution respirometry of isolated mitochondria from adult Octopus maya (Class: Cephalopoda) systemic heart. https://doi.org/10.1371/journal.pone.0273554
Serna 2022 MitoFit2022Serna JDC, Ramos VM, Cabral-Costa JV, Vilas-Boas EA, Amaral AG, Ohya G, da Silva CCC, Kowaltowski AJ (2022) Measuring mitochondrial Ca2+ efflux in isolated mitochondria and permeabilized cells. https://doi.org/10.26124/mitofit:2022-0021 — 2022-07-28 published in Bioenerg Commun 2022.7.
Mihajlovic 2022 Int J Mol Sci2022Mihajlovic M, Vinken M (2022) Mitochondria as the target of hepatotoxicity and drug-induced liver injury: molecular mechanisms and detection methods. https://doi.org/10.3390/ijms23063315
Horwath 2022 J Physiol2022Horwath O, Edman S, Andersson A, Larsen FJ, AprĂł W (2022) THRIFTY: a novel high-throughput method for rapid fibre type identification of isolated skeletal muscle fibres. https://doi.org/10.1113/jp282959
Goudie 2022 Sci Rep2022Goudie L, Mancini NL, Shutt TE, Holloway GP, Mu C, Wang A, McKay DM, Shearer J (2022) Impact of experimental colitis on mitochondrial bioenergetics in intestinal epithelial cells. https://doi.org/10.1038/s41598-022-11123-w
Formiga-Jr 2022 J Vis Exp2022Formiga-Jr MA, Camacho-Pereira J (2022) Assessing mitochondrial function in sciatic nerve by high-resolution respirometry. https://doi.org/10.3791/63690
Serna 2022 BEC2022Serna JDC, Ramos VM, Cabral-Costa JV, Vilas-Boas EA, Amaral AG, Ohya G, da Silva CCC, Kowaltowski AJ (2022) Measuring mitochondrial Ca2+ efflux in isolated mitochondria and permeabilized cells. Bioenerg Commun 2022.7. https://doi.org/10.26124/bec:2022-0007
Zdrazilova 2022 PLOS ONE2022Zdrazilova L, Hansikova H, Gnaiger E (2022) Comparable respiratory activity in attached and suspended human fibroblasts. PLoS ONE 17:e0264496. https://doi.org/10.1371/journal.pone.0264496
Mas-Bargues 2022 Antioxidants (Basel)2022Mas-Bargues C, GarcĂ­a-DomĂ­nguez E, BorrĂĄs C (2022) Recent approaches to determine static and dynamic redox state-related parameters. https://doi.org/10.3390/antiox11050864
Baglivo 2022 BEC2022Baglivo E, Cardoso LHD, Cecatto C, Gnaiger E (2022) Statistical analysis of instrumental reproducibility as internal quality control in high-resolution respirometry. Bioenerg Commun 2022.8. https://doi.org/10.26124/bec:2022-0008
Crowder 2022 Islets2022Crowder JJ, Zeng Z, Novak AN, Alves NJ, Linnemann AK (2022) Stabilization protects islet integrity during respirometry in the Oroboros Oxygraph-2K analyzer. Islets 14:128-38.
MiPNet24.12 NextGen-O2k: Q-Module2021-10-29
O2k-Manual
NextGen-O2k: Q-Module manual
MiPNet26.11 NextGen-O2k: PB-Module2021-10-29
O2k-Manual
NextGen-O2k: PB-Module manual
MiPNet24.16 DatLab8.0: CV-Module2021-10-28
O2k-Manual
DatLab8.0: Cyclic voltammetry manual
MiPNet24.10 H2O2 flux analysis2021-10-22
O2k-Protocols
Hydrogen peroxide flux analysis using Amplex UltraRed assay in MiR05-Kit with DatLab 7.4
MiPNet24.09 Data analysis of mt-membrane potential2021-09-16
O2k-Protocols
Data analysis of mt-membrane potential.
MiPNet26.10 MgG data analysis2021-09-16
O2k-Protocols
Measurement of mitochondrial ATP production using Magnesium Green: DL-Protocols and data analysis with DatLab 7.4
MiPNet15.03 O2k-MultiSensor-ISE2021-08-02
O2k-Manual
O2k-MultiSensor system with ion selective electrodes (ISE).
MiPNet12.10 TIP2k-manual2021-07-08
O2k-Manual
Titration-Injection microPump TIP2k manual.
MiPNet19.18B POS-service2021-06-23
O2k-Manual
Service of the polarographic oxygen sensor OroboPOS.
MiPNet17.05 O2k-Fluo LED2-Module2021-06-22
O2k-Manual
O2k-Fluo LED2-Module.
MiPNet26.06 DatLab 7: Guide2021-06-02
O2k-Manual
DatLab-guide through the menus.
MiPNet20.04 O2k-checklist2021-03-12
O2k-Manual
O2k-checklist: get started with an O2k-experiment.
Gnaiger 2021 MitoFit BCA2021Gnaiger E (2021) Bioenergetic cluster analysis – mitochondrial respiratory control in human fibroblasts. MitoFit Preprints 2021.08. https://doi.org/10.26124/mitofit:2021-0008
Schmidt 2021 J Biol Chem2021Schmidt CA, Fisher-Wellman KH, Neufer PD (2021) From OCR and ECAR to energy: perspectives on the design and interpretation of bioenergetics studies. J Biol Chem 207:101140. https://doi.org/10.1016/j.jbc.2021.101140
Went 2021 MitoFit PB2021Went N, Di Marcello M, Gnaiger E (2021) Oxygen dependence of photosynthesis and light-enhanced dark respiration studied by High-Resolution PhotoRespirometry. MitoFit Preprints 2021.05. https://doi.org/10.26124/mitofit:2021-0005
Komlodi 2021 BEC AmR-O22021KomlĂłdi T, Sobotka O, Gnaiger E (2021) Facts and artefacts on the oxygen dependence of hydrogen peroxide production using Amplex UltraRed. Bioenerg Commun 2021.4. https://doi.org/10.26124/bec:2021-0004
Jedlicka 2021 Physiol Res2021Jedlička J, Kunc R, Kuncová J (2021) Mitochondrial respiration of human platelets in young adult and advanced age - Seahorse or O2k? Physiol Res 70:369-79.
Komlodi 2021 BEC Q2021KomlĂłdi T, Cardoso LHD, Doerrier C, Moore AL, Rich PR, Gnaiger E (2021) Coupling and pathway control of coenzyme Q redox state and respiration in isolated mitochondria. Bioenerg Commun 2021.3. https://doi.org/10.26124/bec:2021-0003
Hoppel 2021 Cells2021Hoppel F, Garcia-Souza LF, Kantner-Rumplmair W, Burtscher M, Gnaiger E, Pesta D, Calabria E (2021) Human platelet mitochondrial function reflects systemic mitochondrial alterations: a protocol for application in field studies. https://doi.org/10.3390/cells10082088
Cardoso 2021 BEC MgG2021Cardoso LHD, Doerrier C, Gnaiger E (2021) Magnesium Green for fluorometric measurement of ATP production does not interfere with mitochondrial respiration. Bioenerg Commun 2021.1. https://doi.org/10.26124/bec:2021-0001
Vernerova 2021 Biomedicines2021Vernerova A, Garcia-Souza LF, Soucek O, Kostal M, Rehacek V, Krcmova LK, Gnaiger E, Sobotka O (2021) Mitochondrial respiration of platelets: comparison of isolation methods. https://doi.org/10.3390/biomedicines9121859
Siewiera 2021 Int J Mol Sci2021Siewiera K, Labieniec-Watala M, Wolska N, Kassassir H, Watala C (2021) Sample preparation as a critical aspect of blood platelet mitochondrial respiration measurements - The impact of platelet activation on mitochondrial respiration. https://doi.org/10.3390/ijms22179332
MiPNet21.17 BloodCellsIsolation2020-10-22
O2k-Protocols
O2k-Protocols: Isolation of blood cells for HRR.
MiPNet24.08 Safranin Analysis Template2020-05-13
O2k-Protocols
Excel template for safranin data analysis.
MiPNet17.04 CitrateSynthase2020-04-18
O2k-Protocols
Laboratory protocol: Citrate synthase a mitochondrial marker enzyme.
MiPNet24.06 Oxygen flux analysis - DatLab 7.42020-04-16
O2k-Protocols
Oxygen flux analysis with DatLab 7.4.
MiPNet24.14 O2k-sV-Module manual2020-03-17
O2k-Manual
O2k-sV-Module manual.
MiPNet25.14 TPP Analysis Template2020-##-##
O2k-Protocols
Excel template for TPP data analysis.
MitoEAGLE muscle 12020Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2020) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. - Updated: 2020-03-08
Gnaiger 2020 BEC MitoPathways2020Gnaiger E (2020) Mitochondrial pathways and respiratory control. An introduction to OXPHOS analysis. 5th ed. Bioenerg Commun 2020.2. https://doi.org/10.26124/bec:2020-0002
Pola 2020 Invest New Drugs2020Pola M, Kolarova H, Ruzicka J, Zholobenko A, Modriansky M, Mosinger J, Bajgar R (2020) Effects of zinc porphyrin and zinc phthalocyanine derivatives in photodynamic anticancer therapy under different partial pressures of oxygen in vitro. Invest New Drugs 39:89-97.
Fisher 2020 Placenta2020Fisher J, McKeating D, Pennell E, Cuffe J, Holland O, Perkins A (2020) Mitochondrial isolation, cryopreservation and preliminary biochemical characterisation from placental cytotrophoblast and syncytiotrophoblast. Placenta 82:1-4.
McCann 2020 Metabolites2020McCann MR, McHugh CE, Kirby M, Jennaro TS, Jones AE, Stringer KA, Puskarich MA (2020) A multivariate metabolomics method for estimating platelet mitochondrial oxygen consumption rates in patients with sepsis. Metabolites 10:E139.
Boehmert 2020 Transfusion2020Böhmert S, KĂŒbel S, MĂŒller MM, Weber CF, Adam EH, Dröse S, Zacharowski K, Fischer D (2020) The effect of the interruption of agitation, temporary cooling, and pneumatic tube transportation on platelet quality during storage for transfusion. Transfusion 61:1258-65.
D'Urso 2020 Surg Endosc2020D'Urso A, Agnus V, Barberio M, Seeliger B, Marchegiani F, Charles AL, Geny B, Marescaux J, Mutter D, Diana M (2020) Computer-assisted quantification and visualization of bowel perfusion using fluorescence-based enhanced reality in left-sided colonic resections. Surg Endosc 35:4321-31.
Dawid 2020 Biochim Biophys Acta Bioenerg2020Dawid C, Weber D, Musiol E, Janas V, Baur S, Lang R, Fromme T (2020) Comparative assessment of purified saponins as permeabilization agents during respirometry. Biochim Biophys Acta Bioenerg 1861:148251.
Assis-de-Lemos 2020 Curr Protoc Toxicol2020Assis-de-Lemos G, Ledur PF, Karmirian K, Rehen SK, Galina A (2020) A protocol to study mitochondrial function in human neural progenitors and iPSC-derived astrocytes. Curr Protoc Toxicol 85:e97.
Injarabian 2020 Sci Rep2020Injarabian L, Scherlinger M, Devin A, Ransac S, Lykkesfeldt J, Marteyn BS (2020) Ascorbate maintains a low plasma oxygen level. Sci Rep 10:10659.
McLaughlin 2020b Sci Rep2020McLaughlin KL, Hagen JT, Coalson HS, Nelson MAM, Kew KA, Wooten AR, Fisher-Wellman KH (2020) Novel approach to quantify mitochondrial content and intrinsic bioenergetic efficiency across organs. Sci Rep 10:17599.
Nolfi-Doneganab 2020 Redox Biol2020Nolfi-Doneganab D, Braganzaa A, Shivaac S (2020) Mitochondrial electron transport: oxidative phosphorylation, mitochondrial oxidant production, and methods of measurement. Redox Biol 2020:101674.
Damiani 2020 Curr Opin Biotechnol2020Damiani C, Gaglio D, Sacco E, Alberghina L, Vanoni M (2020) Systems metabolomics: from metabolomic snapshots to design principles. Curr Opin Biotechnol 63:190-199.
Romeiro 2020 bioRxiv2020Romeiro NC, Ferreira CM, Oliveira MF (2020) Assessment of mitochondrial physiology of murine white adipose tissue by mechanical permeabilization and lipid depletion. bioRxiv doi: https://doi.org/10.1101/2020.02.19.956870.
Huete-Ortega 2020 MitoFit Preprint Arch EA2020Huete-Ortega M, Di Marcello M, Iglesias-Gonzalez J, Gnaiger E (2020) High-resolution respirometry for chloroplast and mitochondrial bioenergetics in Chlamydomonas reinhardtii ― towards biotechnology exploitations. https://doi.org/10.26124/mitofit:ea20.algaeurope.0001
Allen 2020 Thesis2020Allen FM (2020) Mitochondrial metabolism elucidated by rapid fractionation from tissue. PhD Thesis 298.
Madeo 2020 US Patent2020Madeo F, Eisenberg T, Stekovic S (2020) Use of spermidine for the enhancement of mitochondrial respiration. US Patent US20200054579 A1.
MiPNet21.16 DatLab 7 Innovations2019-09-23
About Oroboros
DatLab 7: innovations.
MiPNet22.16 DatLab 7 Innovations: DL-Protocols2019-09-13
O2k-Protocols
DatLab 7.4 Innovations: DL-Protocols
MiPNet23.12 DL-Protocols help2019-09-13
O2k-Protocols
DatLab Protocols: Help DatLab 7.
MiPNet24.11 mtMP calculation2019-08-05
O2k-Protocols
Mitochondrial membrane potential calculation
MiPNet20.14 AmplexRed H2O2-production2019-06-24
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of H2O2 production with Amplex UltraRed.
MiPNet20.13 Safranin mt-membranepotential2019-06-24
O2k-Protocols
O2k-FluoRespirometry: HRR and simultaneous determination of mt-membrane potential with safranin or TMRM.
Di Marcello 2019 MitoFit Preprint Arch EA2019Di Marcello M, Iglesias-Gonzalez J, Meszaros A, Haider M, Gnaiger E, Huete-Ortega M (2019) Partitioning between cytochrome c oxidase and alternative oxidase studied by oxygen kinetics of dark respiration in Chlamydomonas reinhardtii: a microalgae model organism. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0005.v3
Braganza 2019 Mol Aspects Med2019Braganza A, Annarapu GK, Shiva S (2019) Blood-based bioenergetics: An emerging translational and clinical tool. Mol Aspects Med 2019:100835.
Bettink 2019 Mitochondrion2019Bettink MAW, Harms FA, Dollee N, Specht PAC, Raat NJH, Schoonderwoerd GC, Mik EG (2019) Non-invasive versus ex vivo measurement of mitochondrial function in an endotoxemia model in rat: toward monitoring of mitochondrial therapy. Mitochondrion 50:149-57.
Doerrier 2019 MitoFit Preprint Arch EA2019Doerrier C, Gama-Perez P, Distefano G, Pesta D, Soendergaard SD, Chroeis KM, Gonzalez-Franquesa A, Goodpaster BH, Coen P, Larsen S, Gnaiger E, Garcia-Roves PM (2019) Inter-laboratory harmonization of respiratory protocols in permeabilized human muscle fibers. https://doi.org/10.26124/mitofit:ea19.MiPSchool.0009
Naszai 2019 Sci Rep2019NĂĄszai A, Terhes E, Kaszaki J, Boros M, JuhĂĄsz L (2019) Ca(2+)N it be measured? Detection of extramitochondrial calcium movement with high-resolution FluoRespirometry. Sci Rep 9:19229.
Germeys 2019 Genes (Basel)2019Germeys C, Vandoorne T, Bercier V, Van Den Bosch L (2019) Existing and emerging metabolomic tools for ALS research. Genes (Basel) 10:E1011.
Chabi 2019 MitoFit Preprint Arch EA2019Chabi BĂ©atrice, Ost M, Gama-Perez P, Dahdah N, Lemieux H, Holody CD, Carpenter RG, Tepp K, Puurand M, Kaambre T, Dubouchaud H, Cortade F, Pesta D, Calabria E, Casado M, Fernandez-Ortiz M, Acuña-Castroviejo D, Villena JA, Grefte S, Keijer J, O'Brien K, Sowton A, Murray AJ, Campbell MD, Marcinek DJ, Nollet E, WĂŒst R, Dayanidhi S, Gnaiger E, Doerrier C, Garcia-Roves PM (2019) Generating reference values on mitochondrial respiration in permeabilized muscle fibers. https://doi.org/10.26124/mitofit:ea19.MitoEAGLE.0001
Long 2019 Methods Mol Biol2019Long Q, Huang L, Huang K, Yang Q (2019) Assessing mitochondrial bioenergetics in isolated mitochondria from mouse heart tissues using Oroboros Oxygraph-2k. Methods Mol Biol 1966:237-46.
Gvozdjakova 2019 Bratisl Lek Listy2019Gvozdjakova A, Sumbalova Z, Kucharska J, Chladekova A, Rausova Z, Vancova O, Komlosi M, Ulicna O, Mojto V (2019) Platelet mitochondrial bioenergetic analysis in patients with nephropathies and non-communicable diseases: a new method. Bratisl Lek Listy 120:630-35.
MiPNet23.15 O2k-pH ISE-Module2018-09-08
O2k-Manual
Measurement of proton flux with the O2k-pH ISE-Module.
MiPNet21.06 SUIT RP2018-06-25
O2k-Protocols
SUIT reference protocol for OXPHOS analysis by high-resolution respirometry.
Fisher-Wellman 2018 Cell Rep2018Fisher-Wellman KH, Davidson MT, Narowski TM, Lin CT, Koves TR, Muoio DM (2018) Mitochondrial diagnostics: A multiplexed assay platform for comprehensive assessment of mitochondrial energy fluxes. Cell Rep 24:3593-606.
Komlodi 2018 Methods Mol Biol2018Komlodi T, Sobotka O, Krumschnabel G, Bezuidenhout N, Hiller E, Doerrier C, Gnaiger E (2018) Comparison of mitochondrial incubation media for measurement of respiration and hydrogen peroxide production. Methods Mol Biol 1782:137-55.
Havird 2018 bioRxiv2018Havird JC, Noe GR, Link L, Torres A, Logan DC, Sloan DB, Chicco AJ (2018) Assessing mitochondrial function in angiosperms with highly divergent mitochondrial genomes. bioRxiv doi: https://doi.org/10.1101/448902.
Van Daele 2018 Medicine (Baltimore)2018Van Daele E, Van Nieuwenhove Y, Ceelen W, Vanhove C, Braeckman BP, Hoorens A, Van Limmen J, Varin O, Van de Putte D, Willaert W, Pattyn P (2018) Assessment of graft perfusion and oxygenation for improved outcome in esophageal cancer surgery: Protocol for a single-center prospective observational study. Medicine (Baltimore) 97:e12073.
Simard 2018 J Vis Exp2018Simard CJ, Pelletier G, Boudreau LH, Hebert-Chatelain E, Pichaud N (2018) Measurement of mitochondrial oxygen consumption in permeabilized fibers of Drosophila using minimal amounts of tissue. J Vis Exp 134.
Smirnova 2018 Sci Rep2018Smirnova IA, Ädelroth P, Brzezinski P (2018) Extraction and liposome reconstitution of membrane proteins with their native lipids without the use of detergents. Sci Rep 8:14950.
Puurand 2018 Int J Mol Sci2018Puurand M, Tepp K, Klepinin A, Klepinina L, Shevchuk I, Kaambre T (2018) Intracellular energy-transfer networks and high-resolution respirometry: a convenient approach for studying their function. Int J Mol Sci 19:e2933.
Ramil 2018 Thesis2018Ramil LR, Gulbinas A, Cesna V (2018) “HIPEC” hyperthermic intraperitoneal chemotherapy. Evaluation of intraperitoneal free cancer cells. Master's Thesis.
Doerrier 2018 Methods Mol Biol2018Doerrier C, Garcia-Souza LF, Krumschnabel G, Wohlfarter Y, MĂ©szĂĄros AT, Gnaiger E (2018) High-Resolution FluoRespirometry and OXPHOS protocols for human cells, permeabilized fibers from small biopsies of muscle, and isolated mitochondria. Methods Mol Biol 1782:31-70. https://doi.org/10.1007/978-1-4939-7831-1_3
Deschaseaux 2018 US Patent2018Deschaseaux F, Guilloton F, Muller S, Sensebe L, Casteilla L, Carriere-Pazat A (2018) Method for obtaining human brown/beige adipocytes . US Patent US20180216070A1.
Dias 2018 Anal Biochem2018Dias C, Lourenco CF, Barbosa RM, Laranjinha J, Ledo A (2018) Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices. Anal Biochem 551:43-50.
Fischer 2018 BMC Musculoskelet Disord2018Fischer MJ, Horvath G, Krismer M, Gnaiger E, Goebel G, Pesta DH (2018) Evaluation of mitochondrial function in chronic myofascial trigger points - a prospective cohort pilot study using high-resolution respirometry. BMC Musculoskelet Disord 19:388.
Run DL-Protocol/Set O2 limit2017-08-10
MitoPedia
Online documentation of DL-Protocol function of DatLab7.
Djafarzadeh 2017 J Vis Exp2017Djafarzadeh S, Jakob SM (2017) High-resolution respirometry to assess mitochondrial function in permeabilized and intact cells. J Vis Exp 8.doi: 10.3791/54985..
Vienne 2017 Anal Biochem2017Vienne JC, Cimetta C, Dubois M, Duburcq T, Favory R, Dessein AF, Fontaine M, Joncquel-Chevalier Curt M, Cuisset JM, Douillard C, Mention-Mulliez K, Dobbelaere D, Vamecq J (2017) A fast method for high resolution oxymetry study of skeletal muscle mitochondrial respiratory chain complexes. Anal Biochem 528:57-62.
Westerlund 2017 Pediatr Res2017Westerlund E, Marelsson SE, Ehinger JK, Sjövall F, Morota S, Åsander Frostner E, Oldfors A, Darin N, Lundgren J, Hansson MJ, Fellman V, ElmĂ©r E (2017) Oxygen consumption in platelets as an adjunct diagnostic method for pediatric mitochondrial disease. Pediatr Res 83:455-65.
Djafarzadeh 2017b J Vis Exp2017Djafarzadeh S, Jakob SM (2017) Isolation of intact mitochondria from skeletal muscle by differential centrifugation for High-Resolution Respirometry measurements. J Vis Exp doi: 10.3791/55251.
MiPNet21.14 Reference sample HRR2016-10-19
O2k-Protocols
Development of a reference sample for HRR.
MiPNet21.13 Comparison mt-preparations2016-10-18
O2k-Protocols
O2k-Protocols: Mitochondrial preparations for HRR.
MiPNet21.12 Comparison mt respiration media2016-10-18
O2k-Protocols
O2k-MultiSensor: Mitochondrial respiration media for HRR and simultaneous O2k-Fluorometry.
MiPNet19.18C DatLab 6: Guide2016-08-24
O2k-Manual
DatLab-guide through the menus.
MiPNet19.18D O2k-Series G and DatLab 6: Calibration2016-08-24
O2k-Protocols
O2k-calibration by DatLab 6.
MiPNet08.09 CellRespiration2016-08-11
O2k-Procedures contents
High-resolution respirometry and coupling-control protocol with living cells: ROUTINE, LEAK, ET-pathway, ROX.
MiPNet19.18A O2k-Series G: Start2016-08-08
O2k-Manual
Oxygraph-2k: start high-resolution respirometry.
MiPNet19.18 O2k-Series G Core manual2016-08-08
O2k-Manual
O2k-Core manual contents.
MiPNet19.18E O2 flux analysis - DatLab 62016-08-08
O2k-Manual
Oxygen flux analysis: DatLab real-time.
MiPNet10.04 CellRespiration2016-08-08
O2k-Protocols
An experiment with high-resolution respirometry: coupling control in cell respiration.
MiPNet21.07 MitoFit DatLab PT2016-07-20
O2k-Protocols
MitoFit DatLab proficiency test.
MiPNet15.05 NO-manual2016-04-21
O2k-Manual
O2k-MultiSensor system with amperometric sensors: NO, H2S, H2O2.
Reich 2016 Thesis2016Reich V (2016) Pathophysiologie und Verfahrensentwicklung: Mechanismen der Metabolisierung von Schwefelwasserstoff im mitochondrialen Atmungskreislauf von Lungenalveolarmakrophagen. Dissertation p91.
Bai 2016 PLOS ONE2016Bai F, Fink BD, Yu L, Sivitz WI (2016) Voltage-dependent regulation of Complex II energized mitochondrial oxygen flux. PLOS ONE 11:e0154982.
Jang 2016 J Intensive Care Med2016Jang DH, Greenwood JC, Spyres MB, Eckmann D (2016) Measurement of mitochondrial respiration and motility in acute care: sepsis, trauma, and poisoning. J Intensive Care Med 32:86-94.
Chowdhury 2015 Biosci Rep2016Chowdhury SK, Djordjevic J, Albensi B, Fernyhough P (2015) Simultaneous evaluation of substrate-dependent oxygen consumption rates and mitochondrial membrane potential by TMRM and safranin in cortical mitochondria. Biosci Rep 36:e00286.
Stier 2016 Methods Ecol Evol2016Stier A, Romestaing C, Schull Q, Lefol E, Robin JP, Roussel D, Bize P (2016) How to measure mitochondrial function in birds using red blood cells: a case study in the king penguin and perspectives in ecology and evolution. Methods Ecol Evol doi:10.1111/2041-210X.12724.
Ojuka 2016 Am J Physiol Endocrinol Metab2016Ojuka E, Andrew B, Bezuidenhout N, George S, Maarman G, Madlala HP, Mendham A, Osiki PO (2016) Measurement of ÎČ-oxidation capacity of biological samples by respirometry: a review of principles and substrates. Am J Physiol Endocrinol Metab 310:E715-23. https://doi.org/10.1152/ajpendo.00475.2015
Fisar 2016a Folia Biol (Praha)2016Fisar Z, Hroudova J (2016) Pig brain mitochondria as a biological model for study of mitochondrial respiration. Folia Biol (Praha) 62:15-25.
Salin 2016 Physiol Rep2016Salin K, Villasevil EM, Auer SK, Anderson GJ, Selman C, Metcalfe NB, Chinopoulos C (2016) Simultaneous measurement of mitochondrial respiration and ATP production in tissue homogenates and calculation of effective P/O ratios. Physiol Rep 10.14814/phy2.13007.
Ruas 2016 PLOS ONE2016Ruas JS, Siqueira-Santos ES, Amigo I, Rodrigues-Silva E, Kowaltowski AJ, Castilho RF (2016) Underestimation of the maximal capacity of the mitochondrial electron transport system in oligomycin-treated cells. PLOS ONE 11:e0150967.
Lark 2016 Am J Physiol Cell Physiol2016Lark DS, Torres MJ, Lin CT, Ryan TE, Anderson EJ, Neufer PD (2016) Direct real-time quantification of mitochondrial oxidative phosphorylation efficiency in permeabilized skeletal muscle myofibers. Am J Physiol Cell Physiol 311:C239-45.
Khattar 2016 J Neurosci Methods2016Khattar NK, Yablonska S, Baranov SV, Baranova OV, Kretz ES, Larkin TM, Carlisle DL, Richardson RM, Friedlander RM (2016) Isolation of functionally active and highly purified neuronal mitochondria from human cortex. J Neurosci Methods 263:1-6.
Osiki 2016 FASEB J2016Osiki PO, Ojuka E, Marmaan G (2016) An investigation of the suitability of using octanoylcarnitine together with malate as a substrate combination for assessing Beta-oxidation using High Resolution Respirometry. FASEB J 30: 1015.12 .
Dechandt 2016 Anal Biochem2016Dechandt CRP, Couto-Lima CA, Alberici LC (2016) Triglyceride depletion of brown adipose tissue enables analysis of mitochondrial respiratory function in permeabilized biopsies. Anal Biochem 515:55-60.
MiPNet17.03 Shredder vs Fibers2015-06-18
O2k-Protocols
Mitochondrial respiration in permeabilized fibers versus homogenate from fish liver and heart. An application study with the PBI-Shredder.
MiPNet06.06 Chemical O2 background2015-04-27
O2k-Manual
Oxygraph assay of cytochrome c oxidase activity: chemical background correction.
Canto 2015 Curr Protoc Mouse Biol2015CantĂł C, Garcia-Roves PM (2015) High-resolution respirometry for mitochondrial characterization of ex vivo mouse tissues. Curr Protoc Mouse Biol 5:135-53.
Makrecka-Kuka 2015 Biomolecules2015Makrecka-Kuka M, Krumschnabel G, Gnaiger E (2015) High-resolution respirometry for simultaneous measurement of oxygen and hydrogen peroxide fluxes in permeabilized cells, tissue homogenate and isolated mitochondria. https://doi.org/10.3390/biom5031319
Weissig 2015 Methods Mol Biol II2015Weissig V, Edeas M, eds (2015) Mitochondrial medicine: Volume II, Manipulating mitochondrial function. Methods Mol Biol 1265:1-447.
Kake-Guena 2015 J Exp Zool A Ecol Genet Physiol2015Kake-Guena SA, Touisse K, Vergilino R, Dufresne F, Blier PU, Lemieux H1 (2015) Assessment of mitochondrial functions in Daphnia pulex clones using high-resolution respirometry. J Exp Zool A Ecol Genet Physiol 323:292-300.
Cagnone 2015 Methods Mol Biol2015Cagnone G, Vaghjiani V, Lee W, Sun C, Johnson J, Yeung KY, St John JC (2015) Analysis of the mitochondrial DNA and its replicative capacity in induced pluripotent stem cells. Methods Mol Biol 1357:231-67.
Kluckova 2015 Methods Mol Biol2015Kluckova K, Dong L, Bajzikova M, Rohlena J, Neuzil J (2015) Evaluation of respiration of mitochondria in cancer cells exposed to mitochondria-targeted agents. Methods Mol Biol 1265:181-94.
Harrison 2015 J Appl Physiol2015Harrison DK, Fasching M, Fontana-Ayoub M, Gnaiger E (2015) Cytochrome redox states and respiratory control in mouse and beef heart mitochondria at steady-state levels of hypoxia. J Appl Physiol 119:1210-8. https://doi.org/10.1152/japplphysiol.00146.2015
Abou-Hamdan 2015 Methods Enzymol2015Abou-Hamdan A, Guedouari-Bounihi H, Lenoir V, Andriamihaja M, Blachier F, Bouillaud F (2015) Oxidation of H2S in mammalian cells and mitochondria. Methods Enzymol 554:201-28.
Krumschnabel 2015 Methods Mol Biol2015Krumschnabel G, Fontana-Ayoub M, Sumbalova Z, Heidler J, Gauper K, Fasching M, Gnaiger E (2015) Simultaneous high-resolution measurement of mitochondrial respiration and hydrogen peroxide production. Methods Mol Biol 1264:245-61.
MiPNet20.17 MiP2015 Lucni Bouda2015
MiPsociety
Pecina P et al, eds (2015) Mitochondrial physiology conference. MiP2015. Mitochondr Physiol Network 20.17:87 pp.
Wuest 2015 J Physiol2015WĂŒst RC, Helmes M, Stienen GJ (2015) Rapid changes in NADH and flavin autofluorescence in rat cardiac trabeculae reveal large mitochondrial complex II reserve capacity. J Physiol 593:1829-40.
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