Category:Ambiguity crisis - NAD and H+

Hydrogen ion ambiguities in the electron transfer system

Communicated by Gnaiger E (2023-10-08) last update 2023-11-10

Electron (e^-) transfer linked to hydrogen ion (hydron; H⁺) transfer is a fundamental concept in the field of bioenergetics, critical for understanding redox-coupled energy transformations.

However, the current literature contains inconsistencies regarding H⁺ formation on the negative side of bioenergetic membranes, such as the matrix side of the mitochondrial inner membrane, when NADH is oxidized during oxidative phosphorylation (OXPHOS). Ambiguities arise when examining the oxidation of NADH by respiratory Complex I or succinate by Complex II.

Oxidation of NADH or succinate involves a two-electron transfer of 2{H⁺+e^-} to FMN or FAD, respectively. Figures indicating a single electron e^- transferred from NADH or succinate lack accuracy.

The oxidized NAD⁺ is distinguished from NAD indicating nicotinamide adenine dinucleotide independent of oxidation state.

NADH + H⁺ → NAD⁺ +2{H⁺+e^-} is the oxidation half-reaction in this H⁺-linked electron transfer represented as 2{H⁺+e^-} (Gnaiger 2023). Putative H⁺ formation shown as NADH → NAD⁺ + H⁺ conflicts with chemiosmotic coupling stoichiometries between H⁺ translocation across the coupling membrane and electron transfer to oxygen. Ensuring clarity in this complex field is imperative to tackle the apparent ambiguity crisis and prevent confusion, particularly in light of the increasing number of interdisciplinary publications on bioenergetics concerning diagnostic and clinical applications of OXPHOS analysis.

Supplement 1. The CI substrate is NADH + H⁺

In mitochondrial preparations, NADH-generating substrates, N, of various dehydrogenases (pyruvate, glutamate, malate, or other ET-pathway competent N-type substrate combinations) are applied to support respiration through Complex I (Gnaiger 2020). Importantly, malate and other N-type substrates are not CI-substrates.

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Fig. 1 of Bottje (2019): NADH+H⁺ should be indicated as substrate of CI compared to succinate as substrate of CII. Their oxidation is a 2-electron reaction.

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Supplement 2. Electron transfer from CI ⟶ CII ⟶ CIII

The term electron transfer system takes into account the convergent structure of mitochondrial pathways merging from various branched routes at the N-junction and Q-junction (Gnaiger 2020). Unfortunately the term electron transport chain is still used in some branches of the bioenergetic literature. Combined with respiratory Complexes defined in numerical sequence as CI, CII, CIII, and CIV, this led to misrepresenting electron transfer as (NADH, FADH₂) → CI → CII → CIII:

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Supplement 3. NADH + H⁺ ⟶ NAD⁺ + (2)H⁺ (+2e^-)

It is important to distinguish in the oxidation of NADH the H⁺ that is consumed as a substrate (on the left side of the equation) from the 2H⁺ that are donated in the redox reaction of H⁺-linked electron transfer to the reductant FMN:

NADH + H⁺ ⟶ NAD⁺ + 2{H⁺+e^-}

In H⁺-linked electron transfer, the 2e^- should be matched with 2H⁺.

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Fig. 5 of Mathur et al (2017): H⁺ is consumed in the redox chemical (scalar) reaction of H⁺-linked electron transfer catalyzed by CI, NADH + H⁺ → NAD⁺ + 2H⁺. This same H⁺ is shown to be transported in the vectorial H⁺ translocation from the matrix side across the mtIM (H⁺_neg → H⁺_pos). The scalar and vectorial transformations must be distinguished. When the path is shown of the single electron (instead of 2{H⁺+e^-}) from NADH to ubiquinone, then it is particularly confusing when the 2H⁺ in the H⁺-linked electron transfer are indicated as remaining in the matrix.

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Fig. 5 of Rosca et al (2012): Oxidation of succinate to fumarate reduces ubiquinone to ubiquinol through oxidation of FAD to FADH₂ and further redox steps in CII. Likewise, oxidation of NADH + H⁺ to NAD⁺ reduces ubiquinone to ubiquinol through oxidation of FMN to FMNH₂ and further redox steps in CI. This reduction of UQ to UQH₂ effectively consumes the 2H⁺ together with 2e^-. Hence, the 2H⁺ should not be shown to be formed in the matrix. Indication of H⁺-linked electron transfer as 2{H⁺+e^-} eliminates the ambiguity.

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## Copied with permission from: Hall J (2016) Guyton and Hall Textbook of Medical Physiology. 13. Elsevier, Philadelphia.

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Supplement 4. NADH (+H⁺) ⟶ NAD⁺ + e^-

4.1. NADH + H⁺ ⟶ NAD⁺ + e^-

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4.2. NADH ⟶ NAD⁺ + e^-

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4.3. NADH ⟶ e^-

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Supplement 5. NADH ⟶ NAD⁺ + H⁺ (+ 2e^-)

In the chemical equation for oxidation of NADH + H⁺ by CI,

NADH + H⁺ → NAD⁺ + 2{H⁺+e^-}

H⁺ linked to electron transfer is indicated as 2{H⁺+e^-} on the product side, in contrast to H⁺ defined as a substrate in the chemical reaction (Gnaiger 2023). This formal distinction avoids an ambiguity arising from writing this equation as (see above),

NADH + H⁺ → NAD⁺ + 2H⁺

Then H⁺ on both sides of the equation may be considered to cancel, which provides a possible explanation for the frequent occurrence of the erroneous rearrangement as

NADH → NAD⁺ + H⁺

5.1. NADH ⟶ NAD⁺ + H⁺ + 2e^-

Electron flow from NADH to FMN in CI proceeds as a 2e^- transfer. The 2e^- are never free floating in the matrix but belong to the arrow reaching ubiquinone UQ. 2-electron transfer is linked to 2H⁺ transfer, hence the meaning of the single H⁺ — written on the right side of the equation — is elusive.

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5.2. NADH ⟶ NAD⁺ + H⁺ + e^-

The 2-electron transfer from NADH to CI is not well depicted in graphical representations suggesting the equation NADH ⟶ NAD⁺ + H⁺ + e^-.

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Fig. 1 by Głombik et al (2021): Red arrows indicate e^- transfer from NADH through CI to Q and from FADH₂ through CII to Q. In oxidation of NADH, one blue H⁺ appears in the matrix, which is pumped across the mtIM through CI. In fact, reducing equivalents are not pumped but are consumed in the reduction of FMN and further of UQ to UQH₂. 2H⁺ appear in the matrix in oxidation of FADH₂. Their fate is not clear (CII is not a H⁺ pump).

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5.3. NADH ⟶ NAD⁺ + H⁺

In many cases the oxidation NADH ⟶ NAD⁺ + H⁺ by CI is compared with oxidation by CII of succinate to fumarate, succinate to fumarate + 2H⁺, FADH₂ to FAD, or FADH₂ to FAD + 2H⁺. These combinations underscore the ambiguous nature in these portrayals of the transfer of reducing equivalents. What is the meaning of H⁺ in the equation NADH ⟶ NAD⁺ + H⁺ suggested by the following graphical representations of NADH oxidation by CI?

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Fig. 1 by Bejali et al (2020): The meaning of e^- attached to various enzymes is not clear. For indication of electron transfer, corresponding arrows should be added.

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xx Payen VL, Zampieri LX, Porporato PE, Sonveaux P (2019) Pro- and antitumor effects of mitochondrial reactive oxygen species. Cancer Metastasis Rev 38:189-203. - »Bioblast link«

S5.3

xx Penjweini R, Roarke B, Alspaugh G, Gevorgyan A, Andreoni A, Pasut A, Sackett DL, Knutson JR (2020) Single cell-based fluorescence lifetime imaging of intracellular oxygenation and metabolism. Redox Biol 34:101549. - »Bioblast link«

Labelling FAD as 'Free FAD' is incorrect, since the prosthetic group FAD/FADH₂ remains covalently bound to the subunit SDHA of CII in the catalytic cycle.

S5.3

xx Puntel RL, Roos DH, Seeger RL, Rocha JB (2013) Mitochondrial electron transfer chain complexes inhibition by different organochalcogens. Toxicol In Vitro 27:59-70. - »Bioblast link«

S5.3

xx Sazanov LA (2015) A giant molecular proton pump: structure and mechanism of respiratory complex I. Nat Rev Mol Cell Biol 16:375-88. - »Bioblast link«

S5.3

xx Schon EA (2018) Bioenergetics through thick and thin. Science 362:1114-5. - »Bioblast link«

S5.3

xx Srivastava S (2016) Emerging therapeutic roles for NAD(+) metabolism in mitochondrial and age-related disorders. Clin Transl Med 5:25. - »Bioblast link«

S5.3

xx Toleikis A, Trumbeckaite S, Liobikas J, Pauziene N, Kursvietiene L, Kopustinskiene DM (2020) Fatty acid oxidation and mitochondrial morphology changes as key modulators of the affinity for ADP in rat heart mitochondria. Cells 9:340. - »Bioblast link«

S5.3

xx Xing Y (2022) Is genome instability a significant cause of aging? A review. Atlantis Press. - »Bioblast link«

S5.3

xx Yang Y, Wu Y, Sun XD, Zhang Y (2021) Reactive oxygen species, glucose metabolism, and lipid metabolism. Springer In: Huang C, Zhang Y (eds) Oxidative stress. Springer, Singapore. - »Bioblast link«

Fig. 9.1 Arrows are missing from substrates to products, which are required to make this graph meaningful.

S5.3

Supplement 6. NADH ⟶ NAD + H⁺ (+ e^-)

NAD is the IUPAC symbol for nicotinamide adenine dinucleotide without implication of its oxidation state, whereas the oxidized form of NAD is NAD⁺ and the reduced form of NAD is NADH (in terms of total amount, NAD = NADH + NAD⁺).

6.1. NADH ⟶ NAD + H⁺ + e^-

xx Chen TH, Koh KY, Lin KM, Chou CK (2022) Mitochondrial dysfunction as an underlying cause of skeletal muscle disorders. Int J Mol Sci 23:12926. - »Bioblast link«

S6.1

xx Lima A, Lubatti G, Burgstaller J, Hu D, Green AP, Di Gregorio A, Zawadzki T, Pernaute B, Mahammadov E, Perez-Montero S, Dore M, Sanchez JM, Bowling S, Sancho M, Kolbe T, Karimi MM, Carling D, Jones N, Srinivas S, Scialdone A, Rodriguez TA (2021) Cell competition acts as a purifying selection to eliminate cells with mitochondrial defects during early mouse development. Nat Metab 3:1091-108. - »Bioblast link«

S6.1

xx Schniertshauer D, Wespel S, Bergemann J (2023) Natural mitochondria targeting substances and their effect on cellular antioxidant system as a potential benefit in mitochondrial medicine for prevention and remediation of mitochondrial dysfunctions. Curr Issues Mol Biol 45:3911-32. - »Bioblast link«

S6.1

6.2. NADH ⟶ NAD + H⁺

xx Alston CL, Rocha MC, Lax NZ, Turnbull DM, Taylor RW (2017) The genetics and pathology of mitochondrial disease. J Pathol 241:236-50. - »Bioblast link«

S6.2

xx Cuperus R, Leen R, Tytgat GA, Caron HN, van Kuilenburg AB (2010) Fenretinide induces mitochondrial ROS and inhibits the mitochondrial respiratory chain in neuroblastoma. Cell Mol Life Sci 67:807-16. - »Bioblast link«

S6.2

xx Diaz EC, Adams SH, Weber JL, Cotter M, Børsheim E (2023) Elevated LDL-C, high blood pressure, and low peak V˙O2 associate with platelet mitochondria function in children-The Arkansas Active Kids Study. Front Mol Biosci 10:1136975. - »Bioblast link«

S6.2

xx Ježek P, Jabůrek M, Holendová B, Engstová H, Dlasková A (2023) Mitochondrial cristae morphology reflecting metabolism, superoxide formation, redox homeostasis, and pathology. Antioxid Redox Signal. https://doi.org/10.1089/ars.2022.0173 - »Bioblast link«

S6.2

Supplement 7. NADH ⟶ NAD⁺ + 2H⁺ (+ 2e^-)

A bit of bioenergetic mystery is the origin of the form of the chemical reaction supposedly catalyzed by CI,

NADH → NAD⁺ + 2H⁺

If H⁺-linked electron transfer would be indicated, then the 2H⁺ should be directed together with 2e^- to the prosthetic group FMN bound to CI instead of being pushed into the matrix space, and a H⁺ is missing on the substrate side. The unambiguous form of the equation is (Gnaiger 2023),

NADH + H⁺ → NAD⁺ + 2{H⁺+e^-}

7.1. NADH ⟶ NAD⁺ + 2H⁺ + 2e^-

xx Anoar S, Woodling NS, Niccoli T (2021) Mitochondria dysfunction in frontotemporal dementia/amyotrophic lateral sclerosis: lessons from Drosophila models. Front Neurosci 15:786076. - »Bioblast link«

S7.1

xx Brischigliaro M, Zeviani M (2021) Cytochrome c oxidase deficiency. Biochim Biophys Acta Bioenerg 1862:148335. - »Bioblast link«

S7.1

xx Chavda V, Lu B (2023) Reverse electron transport at mitochondrial Complex I in ischemic stroke, aging, and age-related diseases. Antioxidants (Basel) 12:895. - »Bioblast link«

S7.1

xx Cojocaru KA, Luchian I, Goriuc A, Antoci LM, Ciobanu CG, Popescu R, Vlad CE, Blaj M, Foia LG (2023) Mitochondrial dysfunction, oxidative stress, and therapeutic strategies in diabetes, obesity, and cardiovascular disease. Antioxidants (Basel) 12:658. - »Bioblast link«

S7.1

xx Egan B, Sharples AP (2023) Molecular responses to acute exercise and their relevance for adaptations in skeletal muscle to exercise training. Physiol Rev 103:2057-2170. - »Bioblast link«

S7.1

xx Fahimi P, Matta CF (2022) The hot mitochondrion paradox: reconciling theory and experiment. Trends in Chemistry 4:4-20. - »Bioblast link«

S7.1

xx Faria R, Boisguérin P, Sousa Â, Costa D (2023) Delivery systems for mitochondrial gene therapy: a review. Pharmaceutics 15:572. - »Bioblast link«

S7.1

xx Foo J, Bellot G, Pervaiz S, Alonso S (2022) Mitochondria-mediated oxidative stress during viral infection. Trends Microbiol 30:679-92. - »Bioblast link«

S7.1

xx George CE, Saunders CV, Morrison A, Scorer T, Jones S, Dempsey NC (2023) Cold stored platelets in the management of bleeding: is it about bioenergetics? Platelets 34:2188969 - »Bioblast link«

S7.1

xx Gopalakrishnan S, Mehrvar S, Maleki S, Schmitt H, Summerfelt P, Dubis AM, Abroe B, Connor TB Jr, Carroll J, Huddleston W, Ranji M, Eells JT (2020) Photobiomodulation preserves mitochondrial redox state and is retinoprotective in a rodent model of retinitis pigmentosa. Sci Rep 10:20382. - »Bioblast link«

S7.1

xx Hidalgo-Gutiérrez A, González-García P, Díaz-Casado ME, Barriocanal-Casado E, López-Herrador S, Quinzii CM, López LC (2021) Metabolic targets of coenzyme Q10 in mitochondria. Antioxidants (Basel) 10:520. - »Bioblast link«

S7.1

xx Joshi A, Ito T, Picard D, Neckers L (2022) The mitochondrial HSP90 paralog TRAP1: structural dynamics, interactome, role in metabolic regulation, and inhibitors. Biomolecules 12:880. - »Bioblast link«

S7.1

xx Keidar N, Peretz NK, Yaniv Y (2023) Ca²⁺ pushes and pulls energetics to maintain ATP balance in atrial cells: computational insights. Front Physiol 14:1231259. - »Bioblast link«

S7.1

xx Kobayashi A, Takeiwa T, Ikeda K, Inoue S (2023) Roles of noncoding RNAs in regulation of mitochondrial electron transport chain and oxidative phosphorylation. Int J Mol Sci 24:9414. - »Bioblast link«

S7.1

xx Kugler BA, Thyfault JP, McCoin CS (2023) Sexually dimorphic hepatic mitochondrial adaptations to exercise: a mini-review. J Appl Physiol (1985) 134:685-91. - »Bioblast link«

S7.1

xx Lu F (2023) Hypothetical hydrogenase activity of human mitochondrial Complex I and its role in preventing cancer transformation. Explor Res Hypothesis Med 8:280-5. - »Bioblast link«

S7.1

xx Martell E, Kuzmychova H, Kaul E, Senthil H, Chowdhury SR, Morrison LC, Fresnoza A, Zagozewski J, Venugopal C, Anderson CM, Singh SK, Banerji V, Werbowetski-Ogilvie TE, Sharif T (2023) Metabolism-based targeting of MYC via MPC-SOD2 axis-mediated oxidation promotes cellular differentiation in group 3 medulloblastoma. Nat Commun 14:2502. - »Bioblast link«

S7.1

xx Musicco C, Signorile A, Pesce V, Loguercio Polosa P, Cormio A (2023) Mitochondria deregulations in cancer offer several potential targets of therapeutic interventions. Int J Mol Sci 24:10420. - »Bioblast link«

S7.1

xx Nguyen TT, Nguyen DK, Ou YY (2021) Addressing data imbalance problems in ligand-binding site prediction using a variational autoencoder and a convolutional neural network. Brief Bioinform 22:bbab277. - »Bioblast link«

S7.1

xx Prasuhn J, Davis RL, Kumar KR (2021) Targeting mitochondrial impairment in Parkinson's disease: challenges and opportunities. Front Cell Dev Biol 8:615461. - »Bioblast link«

S7.1

xx Shang E, Nguyen TTT, Westhoff MA, Karpel-Massler G, Siegelin MD (2023) Targeting cellular respiration as a therapeutic strategy in glioblastoma. Oncotarget 14:419-25. - »Bioblast link«

S7.1

xx Solhaug A, Gjessing M, Sandvik M, Eriksen GS (2023) The gill epithelial cell lines RTgill-W1, from Rainbow trout and ASG-10, from Atlantic salmon, exert different toxicity profiles towards rotenone. Cytotechnology 75:63-75. - »Bioblast link«

S7.1

xx Turton N, Bowers N, Khajeh S, Hargreaves IP, Heaton RA (2021) Coenzyme Q10 and the exclusive club of diseases that show a limited response to treatment. Expert Opinion Orphan Drugs 9:151-60. - »Bioblast link«

S7.1

xx Vargas-Mendoza N, Angeles-Valencia M, Morales-González Á, Madrigal-Santillán EO, Morales-Martínez M, Madrigal-Bujaidar E, Álvarez-González I, Gutiérrez-Salinas J, Esquivel-Chirino C, Chamorro-Cevallos G, Cristóbal-Luna JM, Morales-González JA (2021) Oxidative stress, mitochondrial function and adaptation to exercise: new perspectives in nutrition. Life (Basel) 11:1269. - »Bioblast link«

S7.1

xx Vesga LC, Silva AMP, Bernal CC, Mendez-Sánchez SC, Bohórquez ARR (2021) Tetrahydroquinoline/4,5-dihydroisoxazole hybrids with a remarkable effect over mitochondrial bioenergetic metabolism on melanoma cell line B16F10. Med Chem Res 30:2127–43. - »Bioblast link«

S7.1

xx Yang Y, Zhang X, Hu X, Zhao J, Chen X, Wei X, Yu X (2022) Analysis of the differential metabolic pathway of cultured Chlorococcum humicola with hydroquinone toxic sludge extract. J Cleaner Production 370:133486. - »Bioblast link«

S7.1

xx Yin M, O'Neill LAJ (2021) The role of the electron transport chain in immunity. FASEB J 35:e21974. - »Bioblast link«

S7.1

7.2. NADH ⟶ NAD⁺ + 2H⁺ + e^-

xx Gallinat A, Vilahur G, Padró T, Badimon L (2022) Network-assisted systems biology analysis of the mitochondrial proteome in a pre-clinical model of ischemia, revascularization and post-conditioning. Int J Mol Sci 23:2087. - »Bioblast link«

S7.2

xx Ignatieva E, Smolina N, Kostareva A, Dmitrieva R (2021) Skeletal muscle mitochondria dysfunction in genetic neuromuscular disorders with cardiac phenotype. Int J Mol Sci 22:7349. - »Bioblast link«

S7.2

7.3. NADH ⟶ NAD⁺ + 2H⁺

xx Dilliraj LN, Schiuma G, Lara D, Strazzabosco G, Clement J, Giovannini P, Trapella C, Narducci M, Rizzo R (2022) The evolution of ketosis: potential impact on clinical conditions. Nutrients 14:3613. - »Bioblast link«

S7.3

xx El-Gammal Z, Nasr MA, Elmehrath AO, Salah RA, Saad SM, El-Badri N (2022) Regulation of mitochondrial temperature in health and disease. Pflugers Arch 474:1043-51. - »Bioblast link«

S7.3

xx Yu-Wai-Man P, Griffiths PG, Chinnery PF (2011) Mitochondrial optic neuropathies - disease mechanisms and therapeutic strategies. Prog Retin Eye Res 30:81-114. - »Bioblast link«

S7.3

Supplement 8. NADH ⟶ NAD + (2H⁺) + (2e^-)

NAD is the IUPAC symbol for nicotinamide adenine dinucleotide without implication of its oxidation state, whereas the oxidized form of NAD is NAD⁺ and the reduced form of NAD is NADH (in terms of total amount, NAD = NADH + NAD⁺).

8.1. NADH ⟶ NAD + 2H⁺ + 2e^-

xx Kataoka N, Matsutani M, Matsushita K (2020) Respiratory chain and energy metabolism of Corynebacterium glutamicum. In: Inui M, Toyoda K (eds) Corynebacterium glutamicum. Microbiology Monographs 23. Springer, Cham. - »Bioblast link«

S8.1

xx Shields HJ, Traa A, Van Raamsdonk JM (2021) Beneficial and detrimental effects of reactive oxygen species on lifespan: a comprehensive review of comparative and experimental studies. Front Cell Dev Biol 9:628157. - »Bioblast link«

S8.1

xx Wu Z, Ho WS, Lu R (2022) Targeting mitochondrial oxidative phosphorylation in glioblastoma therapy. Neuromolecular Med 24:18-22. - »Bioblast link«

S8.1

8.2. NADH ⟶ NAD + 2e^-

xx Yang J, Guo Q, Feng X, Liu Y, Zhou Y (2022) Mitochondrial dysfunction in cardiovascular diseases: potential targets for treatment. Front Cell Dev Biol 10:841523. - »Bioblast link«

S8.2

8.3. NADH ⟶ NAD + e^-

xx Chi SC, Cheng HC, Wang AG (2022) Leber hereditary optic neuropathy: molecular pathophysiology and updates on gene therapy. Biomedicines 10:1930. - »Bioblast link«

S8.3

xx Geng Y, Hu Y, Zhang F, Tuo Y, Ge R, Bai Z (2023) Mitochondria in hypoxic pulmonary hypertension, roles and the potential targets. Front Physiol 14:1239643. - »Bioblast link«

S8.3

xx Simon L, Molina PE (2022) Cellular bioenergetics: experimental evidence for alcohol-induced adaptations. Function (Oxf) 3:zqac039. - »Bioblast link«

S8.3

8.4. NADH ⟶ NAD

xx Beier UH, Angelin A, Akimova T, Wang L, Liu Y, Xiao H, Koike MA, Hancock SA, Bhatti TR, Han R, Jiao J, Veasey SC, Sims CA, Baur JA, Wallace DC, Hancock WW (2015) Essential role of mitochondrial energy metabolism in Foxp3⁺ T-regulatory cell function and allograft survival. FASEB J 29:2315-26. - »Bioblast link«

S8.4

xx Howie D, Waldmann H, Cobbold S (2014) Nutrient sensing via mTOR in T cells maintains a tolerogenic microenvironment. Front Immunol 5:409. - »Bioblast link«

S8.4

xx Murray AJ (2009) Metabolic adaptation of skeletal muscle to high altitude hypoxia: how new technologies could resolve the controversies. Genome Med 1:117. - »Bioblast link«

S8.4

xx Prasun P (2020) Role of mitochondria in pathogenesis of type 2 diabetes mellitus. J Diabetes Metab Disord 19:2017-22. - »Bioblast link«

S8.4

xx Steiner JL, Lang CH (2017) Etiology of alcoholic cardiomyopathy: Mitochondria, oxidative stress and apoptosis. Int J Biochem Cell Biol 89:125-35. - »Bioblast link«

S8.4

xx Tirichen H, Yaigoub H, Xu W, Wu C, Li R, Li Y (2021) Mitochondrial reactive oxygen species and their contribution in chronic kidney disease progression through oxidative stress. Front Physiol 12:627837. - »Bioblast link«

S8.4

xx Wang Peixin, Wang D, Hu J, Tan BK, Zhang Y, Lin S (2021) Natural bioactive peptides to beat exercise-induced fatigue: A review. Food Bioscience 43:101298. - »Bioblast link«

S8.4

Supplement 9. NADH + H⁺ ⟶ NAD + e^-

xx Gopan A, Sarma MS (2021) Mitochondrial hepatopathy: Respiratory chain disorders- 'breathing in and out of the liver'. World J Hepatol 13:1707-26. - »Bioblast link«

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Supplement 10. NADH + H ⟶ NAD⁺ + 2e^-

xx Vartak R, Porras CA, Bai Y (2013) Respiratory supercomplexes: structure, function and assembly. Protein Cell 4:582-90. - »Bioblast link«

Fig. 1 of Vartak et al (2013): The misconstrued charge on FAD in FADH₂ → FAD⁺ may explain the explicit one-electron (1e) transfer shown for the 2-electron transfer from FADH₂.

S10

Supplement 11. NADH + H⁺ ⟶ NADH

xx Cadonic C, Sabbir MG, Albensi BC (2016) Mechanisms of mitochondrial dysfunction in Alzheimer's disease. Mol Neurobiol 53:6078-90. - »Bioblast link«

S11

Supplement 12. NADH₂ ⟶ NAD⁺

xx Papa S, Petruzzella V, Scacco S (2007) Electron transport. Structure, redox-coupled protonmotive activity, and pathological disorders of respiratory chain Complexes. Springer, Boston, MA. In: Lajtha A, Gibson GE, Dienel GA (eds) Handbook of neurochemistry and molecular neurobiology:93–118. - »Bioblast link«

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Supplement 13. NADH ⟶ for CI, but NAD⁺ ⟶ NADH + H⁺ for TCA cycle DH

xx DiMauro S, Rustin P (2009) A critical approach to the therapy of mitochondrial respiratory chain and oxidative phosphorylation diseases. Biochim Biophys Acta 1792:1159-67. - »Bioblast link«

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Supplement 14. Reverse

xx Grandoch M, Flögel U, Virtue S, Maier JK, Jelenik T, Kohlmorgen C, Feldmann K, Ostendorf Y, Castañeda TR, Zhou Z, Yamaguchi Y, Nascimento EBM, Sunkari VG, Goy C, Kinzig M, Sörgel F, Bollyky PL, Schrauwen P, Al-Hasani H, Roden M, Keipert S, Vidal-Puig A, Jastroch M5, Haendeler J, Fischer JW (2019) 4-Methylumbelliferone improves the thermogenic capacity of brown adipose tissue. Nat Metab 1:546-59. - »Bioblast link«

NADH is shown as the product of the reaction catalyzed by CI in respiration. This error is rare in the literature, but comparable to the error frequenty encountered when FADH₂ is shown as the substrate of CII.

S14

xx Hunt KA, Jennings RM, Inskeep WP, Carlson RP (2018) Multiscale analysis of autotroph-heterotroph interactions in a high-temperature microbial community. PLoS Comput Biol 14:e1006431. Nat Metab 1:546-59. - »Bioblast link«

NADH is shown as the product of the reaction catalyzed by CI in respiration.

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xx Zhao H, Li Y (2021) Cancer metabolism and intervention therapy. Mol Biomed 2:5. - »Bioblast link«

S14

Supplement 15. Cytochrome b₆f Complex: NADP⁺ + H⁺ ⟶ NADPH

xx Baniulis D, Yamashita E, Zhang H, Hasan SS, Cramer WA (2008) Structure-function of the cytochrome b6f complex. Photochem Photobiol 84:1349-58. - »Bioblast link«

S15

xx Hasan SS, Cramer WA (2012) On rate limitations of electron transfer in the photosynthetic cytochrome b6f complex. Phys Chem Chem Phys 14:13853-60. - »Bioblast link«

S15

xx Liguori N, Croce R, Marrink SJ, Thallmair S (2020) Molecular dynamics simulations in photosynthesis. Photosynth Res 144:273-95. - »Bioblast link«

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Supplement 16. Other

xx Michelet L, Zaffagnini M, Morisse S, Sparla F, Pérez-Pérez ME, Francia F, Danon A, Marchand CH, Fermani S, Trost P, Lemaire SD (2013) Redox regulation of the Calvin-Benson cycle: something old, something new. Front Plant Sci 4:470. - »Bioblast link«

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xx Lauterbach L, Lenz O, Vincent KA (2013) H₂-driven cofactor regeneration with NAD(P)⁺-reducing hydrogenases. FEBS J 280:3058-68. - »Bioblast link«

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xx Li F, Hinderberger J, Seedorf H, Zhang J, Buckel W, Thauer RK (2008) Coupled ferredoxin and crotonyl coenzyme A (CoA) reduction with NADH catalyzed by the butyryl-CoA dehydrogenase/Etf complex from Clostridium kluyveri. J Bacteriol 190:843-50. - »Bioblast link«

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xx Reeve HA, Lauterbach L, Lenz O, Vincent KA (2015) Enzyme-modified particles for selective biocatalytic hydrogenation by hydrogen-driven NADH recycling. ChemCatChem 7:3480-7. - »Bioblast link«

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XX Liang J, Huang H, Wang S (2019) Distribution, evolution, catalytic mechanism, and physiological functions of the flavin-based electron-bifurcating NADH-dependent reduced ferredoxin: NADP+ oxidoreductase. Front Microbiol 10:373. - »Bioblast link«

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