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- 54 bytes (3 words) - 18:51, 15 May 2019
- 40 bytes (3 words) - 23:34, 28 January 2016
- 40 bytes (3 words) - 23:34, 28 January 2016
- |description='''Blood cell preparation''' (bcp) is one of the key steps in diagnostic protocols. |mitopedia topic=Sample preparation752 bytes (87 words) - 14:51, 29 November 2016
- 32 bytes (4 words) - 15:02, 7 July 2017
- |description='''Preparation of SUIT chemicals''' describes the preparation of chemicals used in Substrate-Uncoupler-Inhibitor Ttitration (SUIT) protoc ...That means that small deviations from the concentrations indicated in the preparation instructions will not have any negative effect on the experiment (this was6 KB (916 words) - 08:50, 17 June 2021
Page text matches
- |description='''Blood cell preparation''' (bcp) is one of the key steps in diagnostic protocols. |mitopedia topic=Sample preparation752 bytes (87 words) - 14:51, 29 November 2016
- ...plasma containing the [[platelet]]s. For further details see [[blood cell preparation]]. |mitopedia topic=Sample preparation440 bytes (59 words) - 13:20, 2 May 2021
- ...//wiki.oroboros.at/index.php/O2k-Manual|O2k-Manual]] PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. |abstract= Draxl A, Eigentler A, Gnaiger E (2015) PBI-Shredder HRR-Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function.1 KB (131 words) - 22:39, 13 February 2020
- ...|180px|right]]'''Forceps\stainless Steel\angular Tip\fine''': for [[tissue preparation]], stainless steel. Two pairs are used particularly for muscle fiber separa688 bytes (78 words) - 12:38, 10 November 2020
- == Preparation of resorufin solutions ==895 bytes (124 words) - 00:24, 18 February 2020
- ...fined by the specifications of the [[population]] and by a specific sample preparation (see [[MitoPedia: Sample preparations]]). # [[Property:Preparation]]507 bytes (64 words) - 05:58, 19 July 2022
- ...ight|180px]]'''Forceps\stainless Steel\straight Tip\sharp''': for [[tissue preparation]], stainless steel, antimagnetic. One pair is recommended for insertion of808 bytes (95 words) - 12:48, 10 November 2020
- ...right|180px]]'''Forceps\stainless Steel\rounded Tip\sharp''': for [[tissue preparation]], stainless steel, antimagnetic. One pair is recommended for placing the t827 bytes (96 words) - 12:39, 10 November 2020
- == MiPMap - Comparative: species, tissue and cells, preparation == |?Preparation1 KB (130 words) - 15:56, 11 September 2014
- == MiPMap - Comparative: species, tissue and cells, preparation == |?Preparation1 KB (156 words) - 17:11, 8 June 2015
- == MiPMap - Comparative: species, tissue and cells, preparation == |?Preparation1 KB (133 words) - 15:59, 11 September 2014
- == MiPMap - Comparative: species, tissue and cells, preparation == |?Preparation1 KB (164 words) - 14:50, 29 November 2016
- ...lian liver cells by differential centrifugation: I. Problems, methods, and preparation of extract. J Exp Med 84:51-89. |abstract=1. Materials and technical procedures involved in the preparation of liver suspensions have been described and discussed.867 bytes (117 words) - 13:25, 27 May 2015
- |?preparation=preparation |?preparation=preparation1 KB (210 words) - 11:28, 8 June 2018
- |?Preparation=Preparation |?Preparation=Preparation1 KB (202 words) - 10:04, 3 June 2020
- |?Preparation=Preparation |?Preparation=Preparation1 KB (203 words) - 10:03, 3 June 2020
- |?Preparation=Preparation |?Preparation=Preparation2 KB (203 words) - 10:02, 3 June 2020
- |?Preparation=Preparation |?Preparation=Preparation1 KB (202 words) - 10:02, 3 June 2020
- |?Preparation=Preparation |?Preparation=Preparation1 KB (202 words) - 10:02, 3 June 2020
- |?Preparation=Preparation |?Preparation=Preparation1 KB (202 words) - 10:03, 3 June 2020
- |?Preparation=Preparation |?Preparation=Preparation1 KB (202 words) - 10:03, 3 June 2020
- |?Preparation=Preparation |?Preparation=Preparation2 KB (206 words) - 10:04, 3 June 2020
- |?Preparation=Preparation |?Preparation=Preparation1 KB (190 words) - 10:02, 3 June 2020
- ...wiera K, Labieniec-Watala M, Wolska N, Kassassir H, Watala C (2021) Sample preparation as a critical aspect of blood platelet mitochondrial respiration measuremen ...elationships were not always apparent suggests that the method of platelet preparation may have a greater impact on mitochondrial respiration than the platelet ac2 KB (315 words) - 18:09, 5 June 2023
- ...y acid only after the preparation is treated with proteolytic enzymes. The preparation also contains non-heme iron, lipid, and protoheme, and the last mentioned i1 KB (199 words) - 11:04, 13 February 2015
- |title=Oroboros (2007) Preparation of permeabilized muscle fibres for diagnosis of mitochondrial respiratory f270 bytes (27 words) - 13:35, 17 October 2017
- ...a statistical problem in application of pfi which is averaged in the thom preparation, oxygen limitation is reduced, smaller amounts of tissue are needed compare |mitopedia topic=Sample preparation2 KB (208 words) - 15:19, 31 August 2020
- ...on='''Biopsy preservation solution''', for preservation of tissue samples, preparation of muscle fibres, and permeabilization with [[saponin]].334 bytes (36 words) - 19:25, 18 April 2016
- {{#ask:[[Category:Publications]] [[Preparation::Oxidase; Biochemical Oxidation]] [[Instrument and method::Oxygraph-2k]]337 bytes (43 words) - 16:15, 5 June 2015
- |mitopedia topic=Sample preparation336 bytes (49 words) - 12:59, 23 May 2020
- '''{{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]][[Preparation::Permeabilized tissue]] |format=count}} O2k-Publications on permeabilized t {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Permeabilized tissue]]2 KB (213 words) - 18:55, 15 May 2019
- '''{{#ask:[[Category:Publications]][[Instrument and method::Oxygraph-2k]][[Preparation::Permeabilized cells]] |format=count}} O2k-Publications on [[permeabilized {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Permeabilized cells]]2 KB (214 words) - 16:05, 20 March 2019
- ...values may be used as technical replicates when obtained from the same mt-preparation in different protocols. |?Preparation=Preparation2 KB (325 words) - 08:35, 25 November 2023
- ==== Preparation without BSA ====2 KB (238 words) - 10:01, 9 November 2020
- :::: '''Preparation of 1 M stock solution:''' :::: '''Preparation of 100 mM stock solution of Snv (from the 1 M stock):'''1 KB (154 words) - 12:08, 27 July 2021
- :::: '''Preparation of 150 mM NaCl stock solution.''' :::: '''Preparation of 1.66 mM BSA stock solution.'''2 KB (245 words) - 17:08, 18 January 2022
- |mitopedia topic=Sample preparation464 bytes (61 words) - 16:10, 20 December 2017
- </div><div><!-- Whenever ethanol is used as a solvent (e.g. for preparation of SUIT chemicals), absolute ethanol is meant: ethanol with a low water con High purity grade ethanol has to be used as a solvent especially for preparation of SUIT chemicals. In Oroboros-lab we use ethanol 'AR-grade' (analytical re1 KB (221 words) - 11:29, 27 May 2021
- ...). Dust mask or eyeshields, faceshields and gloves are recommended by the preparation.</span> ::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O): The commercially1 KB (184 words) - 06:35, 20 April 2023
- |title=Porcine hearts samples preparation: PBI-Shredder or saponin? ...very beginning of the experiment. Our aim was to optimize the heart sample preparation from experimental pigs with minimal mitochondrial damage in the sample and3 KB (450 words) - 15:25, 23 January 2019
- |title=Fowler LR, Richardson LH, Hatefi Y (1962) A rapid method for the preparation of highly purified cytochrome oxidase. Biochim Biophys Acta 64:170-3.467 bytes (64 words) - 11:09, 28 May 2015
- ...ration showed a 3.0-fold higher V for Ca2+ uptake compared to the polytron preparation. A lower apparent affinity for Ca2+ was demonstrated in the intermyofibrill2 KB (297 words) - 11:05, 25 February 2015
- ...em from soluble succinic dehydrogenase and a particulate cytochrome system preparation. Nature 181:1520-2.487 bytes (59 words) - 10:37, 28 May 2015
- |?Preparation=Preparations |?Preparation=Preparations1 KB (148 words) - 09:14, 14 April 2023
- ...residual moisture content should be determined immediately prior to sample preparation for combustion calorimetry and elemental analysis. Results from such analys1 KB (209 words) - 20:31, 22 November 2021
- |title=Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with ref ...chrome c conservation, i.e. cytochrome c per mitochondrial quantity in the preparation relative to that in the tissue, is a most useful test parameter. It is boun2 KB (309 words) - 01:21, 16 February 2022
- ...O2k-Dissection-Set.jpg|180px|right]]'''O2k-Dissection Set''': for [[tissue preparation]], set of 4 pairs of stainless steel, antimagnetic forceps and a pair of sc536 bytes (67 words) - 10:42, 6 October 2020
- |mitopedia topic=Sample preparation458 bytes (63 words) - 13:20, 2 May 2021
- ...th permeabilized fibers, and the PBI-Shredder was successfully tested with preparation of trout liver. |additional=O2k-Demo, Mt-Preparation, Auxiliary, PBI-Shredder, O2k-Core2 KB (216 words) - 16:58, 9 December 2020
- ...naturally ([[intrinsic fluorophores]]) or that may be added to a sample or preparation whereby the fluorescence intensity is proportional to the concentration of529 bytes (67 words) - 14:31, 8 February 2016
- ...h practical illustrations and necessary tables and exhaustive details like preparation of a standard operating procedure and a quality manual. Concept of training2 KB (233 words) - 11:50, 12 March 2024
- ...in a reduced state, and electrons flow from TMPD to cytochrome ''c''. For preparation and titration please visit [[Ascorbate]]. :::* 2021-03-9: The preparation instructions were updated to take into account the volume of the solute (TM5 KB (684 words) - 10:08, 3 May 2024
- '''Preparation of 1 mM TTFB stock solution''' (dissolved in H<sub>2</sub>O):475 bytes (58 words) - 13:06, 12 February 2021
- {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Homogenate]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Homogenate]]1 KB (181 words) - 11:36, 11 November 2016
- {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::SMP]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Preparation::SMP]]1 KB (181 words) - 11:37, 11 November 2016
- {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Chloroplasts]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Chloroplasts]]1 KB (181 words) - 11:37, 11 November 2016
- {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Enzyme]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Enzyme]]1 KB (181 words) - 11:37, 11 November 2016
- :::: 2021-03: Preparation instructions were updated to take the volume of the solute into account (se ::::'''Preparation of 4 M storage and stock solution''' (1 mL, dissolved in H<sub>2</sub>O) f2 KB (337 words) - 17:28, 16 December 2021
- :::: '''Preparation of 100 mM stock solution:''' :::: '''Preparation of 10 mM stock solution of Mnanv (from the 100 mM stock):'''1 KB (174 words) - 12:06, 27 July 2021
- === Preparation of KCN stock === :::: ''' Preparation of 0.5 M KCN stock solution''' (dissolved in H<sub>2</sub>O):3 KB (360 words) - 09:51, 10 June 2021
- ...Motic Microscope.jpg|right|180px]]'''Motic Microscope SMZ-171 TLED''': for preparation of permeabilized fibres; compact and light stereo microscope, Greenough opt637 bytes (78 words) - 16:41, 8 September 2022
- {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Isolated mitochondria]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Isolated mitochondria]]1 KB (172 words) - 11:37, 11 November 2016
- {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact organism]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact organism]]1 KB (172 words) - 11:34, 11 November 2016
- |?Preparation |?Preparation2 KB (332 words) - 13:49, 27 October 2023
- {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact organ]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact organ]]1 KB (172 words) - 11:35, 11 November 2016
- :::: 2021-03: The preparation instructions were updated to take the volume of the solute into account (se ::::'''Preparation of 2 M stock solution''' (1 mL, dissolved in H<sub>2</sub>O):2 KB (309 words) - 12:11, 15 June 2021
- {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Oxidase;biochemical oxidation]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Oxidase;biochemical oxidation]]1 KB (175 words) - 11:38, 11 November 2016
- {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact cells]] {{#ask:[[Category:Abstracts]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact cells]]1 KB (179 words) - 07:59, 15 May 2019
- ...r, from the clinicianβs request and including the examination requisition, preparation of the patient, collection of the primary sample, and transportation to and658 bytes (78 words) - 05:27, 19 July 2022
- ...nstrument and method::Oxygraph-2k]] [[Tissue and cell::Skeletal muscle]] [[Preparation::Permeabilized tissue]] ...nstrument and method::Oxygraph-2k]] [[Tissue and cell::Skeletal muscle]] [[Preparation::Permeabilized tissue]]1 KB (195 words) - 14:52, 29 November 2016
- ...al preparation protocols]] including biopsy sampling, tissue storage, cell preparation protocols, etc.2 KB (222 words) - 06:12, 19 July 2022
- {{#ask:[[MitoPedia topic::Sample preparation]]504 bytes (49 words) - 08:34, 24 January 2016
- ...le=Oliveira MF, Geihs MA, França TFA, Moreira DC, Hermes-Lima M (2018) Is "Preparation for Oxidative Stress" a Case of Physiological Conditioning Hormesis?. Front595 bytes (76 words) - 13:20, 31 May 2019
- ...r N (1934) Studies on cell structure by the freezing-drying method VI. The preparation and properties of mitochondria. Anat Rec 60:449-55.577 bytes (68 words) - 10:41, 6 July 2022
- |?Preparation=Preparations |?Preparation=Preparations2 KB (228 words) - 15:41, 8 June 2020
- |?Preparation=Preparations |?Preparation=Preparations2 KB (227 words) - 15:41, 8 June 2020
- |?Preparation=Preparations |?Preparation=Preparations2 KB (227 words) - 15:41, 8 June 2020
- ...: for [[OroboPOS]] and [[ISE]] membrane application; do not use for tissue preparation.671 bytes (75 words) - 18:02, 9 November 2020
- ...by the scientific question as well as the tissue studied and mitochondrial preparation used. In this session, I will discuss issues to be aware of when selecting2 KB (259 words) - 12:16, 17 October 2018
- |?Preparation=Preparations |?Preparation=Preparations2 KB (227 words) - 15:41, 8 June 2020
- ...notion of high integrity was also supported by the reproducibility of the preparation and the magnitude of the respiratory control ratios and the P/O ratios. The2 KB (348 words) - 17:55, 16 January 2021
- |?Preparation516 bytes (69 words) - 04:49, 19 July 2022
- ...'group''') defines the [[sample type]] of an [[experiment]], before sample preparation. The population (or group) size represents the upper limit of the [[sample506 bytes (65 words) - 05:59, 19 July 2022
- ...Boyer PD (1951) The control of pyruvate oxidation in a cell-free rat heart preparation by phosphate acceptors. Science 114:641-2.607 bytes (76 words) - 17:55, 16 January 2021
- :::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>0) for '''2-mL O2k-cham :::: '''Preparation of 0.5 M stock solution''' (dissolved in H<sub>2</sub>0) for '''0.5-mL O2k-3 KB (357 words) - 11:12, 10 June 2021
- |additional=Mt-Preparation826 bytes (103 words) - 17:08, 13 December 2019
- |mitopedia topic=Sample preparation750 bytes (119 words) - 12:54, 23 May 2020
- |additional=Mt-Preparation912 bytes (116 words) - 17:10, 13 December 2019
- ::: '''Preparation of 25 mM stock solution''' (dissolved in ethanol abs.): ::: '''Preparation of 50 Β΅M stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-cha3 KB (374 words) - 15:07, 6 May 2024
- :::: '''Preparation of stock solution: 112 000 U/mL ''' (dissolved in MiR05) for '''2-mL O2k-ch :::: '''Preparation of stock solution: 28 000 U/mL ''' (dissolved in MiR05) for '''0.5-mL O2k-c4 KB (587 words) - 10:27, 14 July 2023
- |additional=Mt-Preparation, MitoFit 2022 NADH1 KB (127 words) - 10:41, 10 April 2022
- |additional=Mt-Preparation946 bytes (119 words) - 17:09, 13 December 2019
- ...s for the investigation of mitochondrial function, the permeabilized brain preparation enhances the capacity for examining regional differences in mitochondrial r2 KB (303 words) - 17:24, 7 November 2016
- ::Dissertation thesis: The modern trends in liquid chromatography and sample preparation for clinical research788 bytes (94 words) - 14:18, 14 April 2020
- |additional=Mt-Preparation943 bytes (113 words) - 14:17, 10 April 2022
- |?Preparation=Preparation2 KB (319 words) - 07:57, 25 November 2023
- * For preparation of MiR05Cr and application in HRR, see [[MiPNet14.13 Medium-MiR06]]776 bytes (104 words) - 18:46, 15 May 2019
- |mitopedia topic=Sample preparation838 bytes (123 words) - 12:55, 23 May 2020
- ...ations and compared our results with the traditional isolated mitochondria preparation.2 KB (327 words) - 15:22, 20 October 2017
- '''MiR06: Mitochondrial Respiration Medium''' ([[#Preparation of MiR05 (MiR06) stock solution|MiR06]] = [[MiR05]] + [[Catalase]]). == Preparation of MiR05 (MiR06) stock solution ==8 KB (1,272 words) - 10:25, 16 March 2022
- |additional=Mt-Preparation1,012 bytes (128 words) - 16:51, 17 October 2023
- :::: '''Preparation of MiR05Cr or MiR06Cr with 20 mM creatine''':842 bytes (118 words) - 13:12, 12 February 2021
- == Preparation of H<sub>2</sub>O<sub>2</sub> stock solutions == ...ion_of_H2O2_stock_solutions | MiPNet14.13]] for H<sub>2</sub>O<sub>2</sub> preparation.4 KB (646 words) - 12:06, 9 November 2021
- |mitopedia topic=Sample preparation951 bytes (115 words) - 17:08, 26 May 2020
- :::: '''Preparation of 5 mM stock solution''' (dissolved in H<sub>2</sub>O): :::: '''Preparation of 2 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''2-mL O2k-cha3 KB (491 words) - 14:55, 7 February 2024
- ...m impairs mitochondrial respiration and induces DNA release in human heart preparation. https://doi.org/10.1371/journal.pntd.0010523 ...ility. Levels of mitochondrial DNA increased in the medium of cardiac cell preparation incubated with venom of ''Bothrops lanceolatus''.2 KB (319 words) - 14:03, 3 October 2022
- ::::* Sample preparation protocols1 KB (127 words) - 04:50, 19 July 2022
- |mitopedia topic=Sample preparation886 bytes (124 words) - 05:54, 19 July 2022
- ...dria from rat liver was estimated by stereological procedures. In the same preparation, the oxidative activity of the respiratory chain with different substrates2 KB (322 words) - 18:00, 16 January 2021
- ...Ayoub M, Gnaiger E (2012) The PBI-Shredder - an auxiliary HRR-tool for the preparation of tissue homogenates for diagnosis of mitochondrial respiratory function. ...eproducibility of pipetting subsamples from the homogenate. A high-quality preparation of tissue homogenate may represent an optimum compromise for a variety of r5 KB (651 words) - 18:25, 10 January 2022
- ...lications]] [[Instrument and method::O2k-Protocol]] [[Additional label::Mt-Preparation]] |?Preparation2 KB (334 words) - 11:21, 28 March 2024
- * For preparation of MiR06Cr and application in HRR, see [[MiPNet14.13 Medium-MiR06]].979 bytes (135 words) - 18:45, 15 May 2019
- ...itochondrial respiratory capacity. The homogenate is compared with the Pfi preparation. Maximal respiratory capacity was significantly reduced in the homogenate c2 KB (332 words) - 13:07, 27 December 2021
- ...rtant considerations concerning storage of NADH (powder and solutions) and preparation of solutions see the product information from Sigma: [https://www.sigmaaldr ::::'''Preparation of NADH to test the integrity of mtIM''':4 KB (477 words) - 10:41, 4 April 2024
- ::::'''Preparation of 2 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''2-mL O2k-ch :::: '''Preparation of 0.5 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''0.5-mL O2k2 KB (317 words) - 12:07, 15 June 2021
- ::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):981 bytes (130 words) - 17:08, 18 January 2022
- |additional=Mt-Preparation1 KB (132 words) - 16:49, 17 October 2023
- |additional=MitoFit 2021 PT, O2k-Demo, O2k-Core, Mt-Preparation1 KB (135 words) - 13:25, 27 October 2023
- ...on the suitability of a rapid muscle preparation which by-passes this long preparation. For a few seconds only, muscle biopsy from pigs is submitted to gentle hom2 KB (298 words) - 16:27, 13 November 2017
- :::: '''Preparation of 5 mM stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamb :::: '''Preparation of 1.25 mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-3 KB (462 words) - 13:23, 15 March 2022
- ::::'''Preparation of 200 mM stock solution''' (dissolved in ethanol abs.):920 bytes (127 words) - 11:53, 22 June 2021
- :::: '''Preparation of 800 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''2-mL O2k-c :::: '''Preparation of 1000 mM stock solution''' (dissolved in H<sub>2</sub>O) for '''0.5-mL O22 KB (329 words) - 09:20, 10 June 2021
- |additional=Mt-Preparation, MitoFit 2021 CoQ, MitoFit 2021 AmR1 KB (137 words) - 06:51, 20 December 2023
- |mitopedia topic=Sample preparation943 bytes (145 words) - 12:55, 23 May 2020
- |mitopedia topic=Sample preparation1 KB (134 words) - 14:17, 8 June 2020
- :::: Preparation for a successful market launch of the NextGen-O2k. ...iotechnology applications. The final component of WP4 for year one was the preparation for ISO certification and the implementation of new quality control measure2 KB (352 words) - 13:23, 3 April 2022
- |additional=O2k-Demo, Mt-Preparation, MitoEAGLE blood cells reviews, MitoFit 2021 PLT1 KB (147 words) - 12:15, 16 September 2021
- ...ning pinacidil derivative preparation floΡalin [2]. It was shown that this preparation at experimental acute ischemia/reperfusion has pronounced cardioprotective ...KATP) membranes [5], the aim of work was to investigate the effect of this preparation on the functioning of mitochondria, and in particular, on work of mitoKATP3 KB (408 words) - 13:03, 7 March 2020
- * For preparation of MiP03 see [[MiPNet03.02 Chemicals-Media]].879 bytes (115 words) - 16:23, 8 February 2016
- ...the muscle tissue, a requirement for respirometry. To achieve appropriate preparation of the tissue sample, training will be required, which will be provided by ...n with other members of the MitoEAGLE consortium. This will ensure optimal preparation to deal with unforeseen eventualities. I have the privilege to be given the6 KB (854 words) - 11:43, 19 June 2019
- |mitopedia topic=Sample preparation899 bytes (101 words) - 11:02, 10 April 2022
- |additional=Mt-Preparation, MitoFit 2021 CoQ, MitoFit 2021 MgG, MitoFit 2021 AmR1 KB (135 words) - 07:10, 20 December 2023
- |additional=O2k-Demo, O2k-MultiSensor, Mt-Preparation, PBI-Shredder, H2021 KB (151 words) - 12:30, 25 October 2023
- ...r B will be filled accordingly. Chamber B can be set to a different sample preparation than chamber A by selecting another option in the corresponding drop-down m5 KB (713 words) - 15:58, 8 May 2024
- ...hredder SG3.jpg|180px|right]] '''PBI-Shredder SG3''' for tissue homogenate preparation, heavy duty high torque SG3 driver with convertible handle, SG3 base with 31 KB (151 words) - 13:34, 14 April 2021
- |?Preparation1 KB (145 words) - 08:45, 27 June 2019
- == MiPMap - Comparative: species, tissue and cells, preparation == |?Preparation6 KB (739 words) - 14:51, 29 November 2016
- ::::'''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O):990 bytes (130 words) - 11:16, 21 June 2021
- |title=[[File:ChabiB.JPG|left|90px]] Optimization of permeabilized fibers preparation for mitochondrial respiration measurements using Design of Experiments meth To optimize the pf preparation from mouse ''Tibialis anterior'' in our lab, we used the Design of Experime4 KB (507 words) - 15:43, 12 January 2018
- === Cytochrome ''c'' release induced by sample preparation === ...the respiratory complexes. Therefore, experimental runs showing a relevant preparation-induced cytochrome ''c'' release should be excluded from the final data set11 KB (1,496 words) - 11:35, 21 January 2022
- |title=Kanner BI, Serrano R, Kandrach MA, Racker E (1976) Preparation and characterization of homogeneous coupling factor 6 from bovine heart mit1 KB (140 words) - 20:58, 21 May 2024
- ...nsity gradient centrifugation. Compared to submitochondrial particles this preparation contained less than 10% of oligomycin-sensitive ATPase and cytochromes. Whe1 KB (163 words) - 09:54, 27 November 2015
- :::: '''Preparation of 200 mM stock solution''' (dissolved in H<sub>2</sub>O)1 KB (136 words) - 12:57, 26 April 2024
- :::: '''Preparation of 500 mM stock solution''' (dissolved in H<sub>2</sub>O)1 KB (140 words) - 12:59, 26 April 2024
- :::: '''Preparation of 5 mM storage and stock solution''' (dissolved in ethanol abs.): :::: '''Preparation of 0.01 mM stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-ch6 KB (917 words) - 12:57, 29 April 2024
- ::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):1 KB (157 words) - 13:05, 12 February 2021
- :::: '''Preparation of 1 mM stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamb :::: '''Preparation of 0.25 mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-4 KB (569 words) - 10:13, 5 March 2024
- A Power-O2k approach (6 instruments, 12 chambers) and fast tissue-homogenate preparation with the PBI-Shredder will provide the basis for a statistically complete s1 KB (135 words) - 16:17, 28 November 2016
- |abstract=Permeabilized muscle fibers is the most widely used sample preparation technique when assessing mitochondrial function in skeletal and cardiac mus ...separation. These improvements contributed to faster, more accurate sample preparation and increased picture quality. Further studies have been carried out using3 KB (407 words) - 18:45, 10 January 2022
- |?Preparation=Preparation |?Preparation=Preparation6 KB (808 words) - 01:02, 31 December 2020
- :::: 2021-03: The preparation instructions were updated to take the volume of the solute (P) into account :::: '''Preparation of 2 M stock solution''' (200 Β΅L, dissolved in H<sub>2</sub>O) for use in4 KB (628 words) - 11:20, 17 June 2021
- |mitopedia topic=Sample preparation ...(see [[MitoPedia: Sample preparations]]) may be, ''e.g.'', a mitochondrial preparation obtained from an organ, cells suspended from a cell culture dish, a tissue6 KB (956 words) - 16:48, 21 February 2024
- '''Preparation of 5 mg/mL stock solution (dissolved in''' [[Biopsy preservation solution|B1 KB (152 words) - 15:42, 4 April 2024
- ..., Rivera-Ingraham GA, Giraud-Billoud M, Genaro-Mattos TC, Campos ΓG (2015) Preparation for oxidative stress under hypoxia and metabolic depression: Revisiting the ...xidative damage that may happen ultimately during reoxygenation. The term "preparation for oxidative stress" (POS) was coined in 1998 based on such premise. Howev3 KB (444 words) - 12:21, 31 May 2019
- ...eless, each laboratory should adapt the preferred method optimizing sample preparation and assay conditions for linearity and a low signal-to-noise ratio. The rea1 KB (172 words) - 21:30, 16 September 2023
- ...5949 or 2-nonyl-4-hydroxyquinoline-N-oxide, and is stimulated by EDTA. The preparation contains DPNH flavoprotein, cytochromes b and c1, Coenzyme Q and non-heme i1 KB (154 words) - 14:33, 13 November 2017
- ...We describe steps for rat dissection, respirometry calibration, and tissue preparation. We then detail measurement of oxygen respiration and protein concentration1 KB (172 words) - 15:12, 27 September 2023
- |title=Lehr S, Hartwig S, Kotzka J (2021) Preparation of "functional" mitochondria: a challenging business. https://doi.org/10.11 KB (193 words) - 17:28, 24 April 2023
- ::: '''Preparation of 10 mM stock solution''' (dissolved in ethanol abs.) (to run cyclic volta ::: '''Preparation of 1 mM stock solution''' (to measure the Q-redox state)3 KB (441 words) - 22:11, 9 April 2022
- ''Work in progress'' by [[Gnaiger E]] 2020-02-12 linked to a preprint in preparation on [[mitObesity drugs]].1 KB (191 words) - 14:13, 24 September 2023
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m2 KB (221 words) - 10:52, 24 March 2024
- ''Work in progress'' by [[Gnaiger E]] 2020-02-23 linked to a preprint in preparation on [[mitObesity drugs]].1 KB (154 words) - 14:10, 24 September 2023
- ::: '''Preparation of 10 mM DNP stock solution''' (dissolved in H<sub>2</sub>O):1 KB (178 words) - 12:53, 12 February 2021
- ...ition of the [[electron transfer pathway]] (ET pathway) in [[mitochondrial preparation]]s or living cells. Different conditions designated as ROX states (differen ...This may be correct to a large extent, but is not entirely accurate. In a preparation of purified isolated mitochondria, a small but significant ''Rox'' is obser10 KB (1,409 words) - 08:36, 25 November 2023
- |mitopedia topic=Sample preparation1 KB (188 words) - 17:09, 26 May 2020
- ''Work in progress'' by [[Gnaiger E]] 2020-02-18 linked to a preprint in preparation on [[mitObesity drugs]].1 KB (162 words) - 14:09, 24 September 2023
- |mitopedia topic=Sample preparation1 KB (166 words) - 14:29, 11 December 2022
- ''Work in progress'' by [[Gnaiger E]] 2023-09-24 linked to a preprint in preparation on [[MitObesity drugs |mitObesity drugs]] - [[:Category:BME and mitObesity1 KB (156 words) - 17:19, 24 September 2023
- :::: '''Preparation of 4 mM cytochrome ''c'' solution''' (dissolved in H<sub>2</sub>O) for '''2 :::: '''Preparation of 5 mM cytochrome ''c'' solution''' (dissolved in H<sub>2</sub>O) for '''06 KB (844 words) - 09:37, 4 April 2024
- :::: 2021-03: The preparation instructions were updated to take the volume of the solute (Gp) into accoun :::: '''Preparation of 1 M stock solution''' (1 mL, dissolved in H<sub>2</sub>O):5 KB (639 words) - 19:25, 25 August 2023
- :::: 2A: '''Mitochondrial assays in blood cells: Blood cell preparation, cryopreservation, respiration.'''1 KB (219 words) - 11:57, 28 June 2016
- ==== Preparation of CaG solution ==== ::: '''Preparation of 1 mM stock solution''' (dissolved in H<sub>2</sub>O):7 KB (971 words) - 15:33, 2 June 2022
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m2 KB (242 words) - 10:49, 24 March 2024
- |?Preparation=Preparation |?Preparation=Preparation6 KB (875 words) - 10:38, 17 November 2021
- ::::'''Preparation of 1 mM storage and stock solution''' (dissolved in ethanol abs.) for '''2- ::::'''Preparation of 0.25 mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-3 KB (443 words) - 18:23, 9 April 2022
- ...oboros Oxygraph-2k system. The method provides detailed procedures for the preparation of mitochondria and measurement of high-resolution respirometry in response1 KB (191 words) - 18:21, 29 February 2020
- === Preparation ===5 KB (694 words) - 11:51, 17 August 2022
- :::: '''Preparation of 500 U/mL stock solution''' (dissolved in MiR05):2 KB (209 words) - 15:59, 8 May 2024
- ...tency might, therefore, be to introduce contraction inhibitor early in the preparation of the sample. ...th myosin II ATPase inhibitor blebbistatin (bleb) during the entire sample preparation exhibit different JO2 when assayed in standard MiR06 sucrose respiration me6 KB (790 words) - 17:46, 7 November 2016
- ''Work in progress'' by [[Gnaiger E]] 2020-02-12 linked to a preprint in preparation on [[mitObesity drugs]].1 KB (180 words) - 14:14, 24 September 2023
- ...corded in recent DatLab versions. A user-friendly O2-kinetics module is in preparation (DL8).1 KB (203 words) - 15:25, 11 November 2019
- ::: '''Preparation of 1 mM stock solution''' (dissolved in ethanol abs.) for '''2-mL O2k-chamb :::: '''Preparation of 0.25 mM stock solution''' (dissolved in ethanol abs.) for '''0.5-mL O2k-3 KB (475 words) - 10:55, 2 August 2021
- ''Work in progress'' by [[Gnaiger E]] 2020-02-12 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m1 KB (191 words) - 15:33, 23 February 2020
- ...problems of [[solution protocols]] (preparation of chemicals), [[sample]] preparation protocols (such as [[mitochondrial preparations]]), and [[SUIT protocols]].4 KB (599 words) - 16:43, 9 August 2022
- :::: '''Preparation of 50 mM stock solution''' (dissolved in H<sub>2</sub>0):1 KB (199 words) - 12:47, 12 February 2021
- ...mended due to lower ATP impurities, we use this (Merck-Calbiochem) ADP for preparation of 'ADP-Mg' solution in [[AT_Innsbruck_Oroboros |Oroboros Lab]]. :::: '''Preparation of 500 mM ADP stock solution''' (dissolved in H<sub>2</sub>O):7 KB (1,046 words) - 11:53, 2 April 2024
- ...t destroy it. For instance, the wet weight of a permeabilized muscle fibre preparation can be determined based on a specific laboratory protocol (gravimetric assa1 KB (215 words) - 05:09, 19 July 2022
- ''in preparation'' ...abilized cells|permeabilized cells]] (considered a type of [[mitochondrial preparation]]). [[Living cells]] are added to the chamber and then the plasma membrane4 KB (562 words) - 13:52, 31 August 2023
- ...MP, Dolgikh DA, Kruglov AA, Kuprash DV, Skulachev VP, Nedospasov SA (2009) Preparation and characterization of mouse embryonic fibroblasts with K72W mutation in s |info=[http://www.ncbi.nlm.nih.gov/pubmed?term=Preparation%20and%20characterization%20of%20mouse%20embryonic%20fibroblasts%20with%20K72 KB (212 words) - 15:40, 9 November 2016
- :::: '''Preparation of 250 mM stock solution''' (dissolved in H<sub>2</sub>O)2 KB (203 words) - 17:39, 18 January 2022
- ...ondrial dysfunctions. Here we discuss methodological aspects of blood cell preparation, show examples of measurements of mitochondrial respiration in intact and p2 KB (205 words) - 14:42, 5 July 2023
- :::'''Preparation of 5 mM storage solution (dissolved in ethanol)''' :::'''Preparation of 0.2 mM stock solution'''5 KB (698 words) - 15:50, 16 March 2021
- ...sy (isolated mitochondria) or lengthy and time-consuming process of sample preparation (permeabilized muscle fibers). In this study we aimed to develop simple, re ...4 Β΅M antimycin. The technique requires only 20 mg of myocardium and sample preparation takes less than 20 min.3 KB (460 words) - 23:10, 19 October 2018
- |mitopedia topic=Sample preparation2 KB (260 words) - 07:18, 19 July 2022
- |abstract=The preparation from beef heart mitochondria and the properties, of a highly ac2 KB (199 words) - 17:16, 16 January 2021
- ...In line with that, I will be trained how to apply the protocols for hPBMC preparation in order to measure oxidative phosphorylation with a high-resolution respir ...4th to 23th January 2020: training in laboratory techniques for (1) hPBMCs preparation: blood sampling, transport, storage and isolation; (2) training in high-res4 KB (544 words) - 16:16, 16 March 2020
- ::::'''Preparation of 10 mg/mL (8.1 mM) stock solution (dissolved in DMSO)''' for '''2-mL O2k- ::::'''Preparation of 2.5 mg/mL (2.025 mM) stock solution (dissolved in DMSO)''' for '''0.5-mL4 KB (504 words) - 14:10, 5 May 2022
- :::: '''Preparation of 5000 U/mL stock solution''' (dissolved in H<sub>2</sub>O)1 KB (227 words) - 14:20, 7 February 2022
- ...s regarding organelle purity, yield, activity and also the duration of the preparation. The most widely used method for mitochondria isolation remains differentia4 KB (548 words) - 10:56, 18 March 2020
- ...l issues relate to the use of out-dated assays and/or inappropriate sample preparation techniques that confound biochemical redox analysis. After considering each2 KB (214 words) - 14:35, 14 April 2022
- ...shed when succinate oxidation is catalyzed by a succinic oxidase preparation containing no phosphorylating system. The possibility that the reac2 KB (258 words) - 07:39, 20 May 2024
- |title=Marks PW, Maxfield FR (1991) Preparation of solutions with free calcium concentration in the nanomolar range using 12 KB (318 words) - 09:27, 7 May 2020
- ...ate ratio of respiratory enzymes under such conditions was investigated on preparation of rat liver mitochondria. It is important to notice that limiting stage of2 KB (221 words) - 09:43, 3 June 2020
- |description='''Preparation of SUIT chemicals''' describes the preparation of chemicals used in Substrate-Uncoupler-Inhibitor Ttitration (SUIT) protoc ...That means that small deviations from the concentrations indicated in the preparation instructions will not have any negative effect on the experiment (this was6 KB (916 words) - 08:50, 17 June 2021
- ...of PLT respiration to respiration of PBMC fraction was calculated for each preparation from the PLT/PBMC ratio in the PBMC fraction (''N''<sub>PLT</sub>/''N''<sub ...nt parameter in respirometric studies, which should be determined in every preparation for quality control. All cell types have to be counted and O<sub>2</sub> fl6 KB (895 words) - 18:50, 10 January 2022
- :::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O)2 KB (227 words) - 10:25, 21 June 2021
- ...stinguish respiratory chain deficient (RCD) patients from controls. Sample preparation, LC-MS, GC-MS and data processing methods were standardised. The developed2 KB (233 words) - 15:28, 23 February 2015
- ...release from skeletal muscle and cardiac mitochondria: influence of sample preparation. c) Permeabilized fibers may be preferred sample preparation for assessment of H<sub>2</sub>O<sub>2</sub>/O<sub>2</sub> consumed, due to4 KB (631 words) - 13:06, 7 March 2020
- |title=MorrΓ© DJ, MorrΓ© DM (1989) Preparation of mammalian plasma membranes by aqueous two-phase partition. Biotechniques2 KB (252 words) - 22:35, 16 February 2021
- ...In this perspective, we offer guidance for best practices of mitochondrial preparation, protocol selection, and measures to increase reproducibility. The goal of2 KB (257 words) - 18:36, 3 November 2023
- ''in preparation'' ...icated by [[Komlodi T]] (names to be added) (last update 2021-11-25) ''in preparation''4 KB (623 words) - 17:57, 13 December 2023
- ::::'''Preparation of 400 mM stock solution''' (dissolved in H<sub>2</sub>O): ::::'''Preparation of 50 mM solution'''4 KB (585 words) - 19:57, 5 February 2022
- ...nk=http://wiki.oroboros.at/index.php/O2k-Protocols|O2k-Protocols contents]]Preparation of permeabilized muscle fibers for diagnosis of mitochondrial respiratory f |abstract='''Pesta D, Gnaiger E (2015) Preparation of permeabilized muscle fibers for diagnosis of mitochondrial respiratory f11 KB (1,593 words) - 13:15, 10 April 2022
- |abstract=The preparation and some properties of a new flavoprotein (ETF) which specifically catalyze2 KB (271 words) - 09:44, 8 May 2023
- ::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):2 KB (238 words) - 17:08, 18 January 2022
- ''in preparation''2 KB (233 words) - 10:43, 26 January 2024
- ...iopsy (isolated mitochondria) or lengthy, time-consuming process of sample preparation (permeabilized muscle fibers). Moreover, permeabilization of cardiac muscle ...enates previously described by Pecinova et al. [1] on animal models. After preparation of tissue homogenates, we aimed to develop a protocol for measurement of ba6 KB (806 words) - 04:58, 18 March 2019
- Different media for tissue preparation and respiration are used in investigations of mitochondrial function. Initi2 KB (265 words) - 17:38, 17 October 2023
- ...or research findings on anti-fatigue peptides, including the isolation and preparation of anti-fatigue peptides, the widely adopted methods for evaluation of anti2 KB (283 words) - 10:57, 9 November 2023
- ...ined with the TPP+-selective electrode developed in the present study. The preparation and some operational parameters of the electrode were described. The kineti2 KB (280 words) - 00:35, 18 February 2022
- ...be used as a possible marker of in vivo oxidative stress. However, sample preparation has an important effect on the redox status of coenzyme Q10 due to the extr2 KB (297 words) - 13:47, 24 August 2021
- ...e is similar to the biological process that animals engage to store fat in preparation for periods of food shortage.2 KB (272 words) - 11:05, 28 April 2015
- ::::* ETP<sub>H</sub>, electron transport particle (membrane preparation)2 KB (280 words) - 13:03, 3 April 2021
- |abstract=The preparation and properties of an electron transfer particle (ETP) obtained from beef he2 KB (289 words) - 12:51, 28 March 2019
- ...mitochondrial respiration in different type of white adipose tissue sample preparation (e.g. whole WAT homogenate, adipocyte after SVF removal, and permeabilized2 KB (273 words) - 15:28, 14 April 2020
- | {{#ask:[[Category:Publications]] [[Instrument and method::Oxygraph-2k]] [[Preparation::Intact organism]] |format=count}} | {{#ask:[[Category:Publications]] [[Preparation::Intact organism]] |format=count}}62 KB (7,038 words) - 14:31, 22 August 2023
- ::::* Muscle fiber (orthopedic controls or rat model) and preparation technique.2 KB (270 words) - 16:11, 23 January 2019
- ...we evaluated the PBI-Shredder as an auxiliary HRR-tool for a standardized preparation of homogenates from various tissues and species. ...ional diagnosis of mitochondrial performance. The fast and reproducible mt-preparation in tissue homogenates opens up new perspectives for comparative mitochondri6 KB (817 words) - 18:28, 10 January 2022
- ...d in a small glass vial. Then the amount H<sub>2</sub>O of the uncorrected preparation instructions was added with one pipette (between 1 and 2 mL). After dissolv The concentrations prepared until 2021-02 were lower than indicated in the preparation instructions, because the volume of the solute was neglected. In a SUIT exp5 KB (865 words) - 11:01, 28 May 2021
- ::::* the preparation of permeabilized tissue (in particular muscle fibers), tissue homogenates o2 KB (287 words) - 15:23, 5 September 2022
- ...M) is an inhibitor of the succinic oxidase system in the Keilin & Hartree preparation. Its major effect appears to be on the accessibility of both the endogenous2 KB (330 words) - 11:06, 26 February 2015
- ...plete, excluding time spent culturing ''C. elegans'', and includes (i) the preparation of ''C. elegans'' samples, (ii) selection and loading of compounds to be in2 KB (286 words) - 00:58, 10 November 2023
- ::::'''Preparation of 10 mM stock solution''' (dissolved in H<sub>2</sub>O):2 KB (262 words) - 12:04, 10 April 2024
- ...her, these features demonstrated mitochondrial integrity and validated the preparation efficacy. Therefore, the protocol described here increases the possibilitie2 KB (292 words) - 11:16, 7 March 2020
- ...oxidized when exposed to air. We recommend to reduce contact to air during preparation in a closed tube. The effective concentration of a solution of reduced dith :::: '''Preparation of 30 mM dithionite solution for instrumental oxygen background test''' (di9 KB (1,336 words) - 07:33, 13 October 2023
- :::: '''Preparation of 25 mM stock solution''' (dissolved in H<sub>2</sub>O)2 KB (289 words) - 12:57, 26 April 2024
- ...ed by calcium, and when calcium chelators are present in the mitochondrial preparation medium and/or experimental incubation medium, calcium must be added to insu2 KB (287 words) - 09:05, 27 July 2018
- ::::* While at the preparation and printing of this review, we are not aware of any studies that directly2 KB (297 words) - 11:09, 24 September 2023
- :::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O; M.W. = 115.13)2 KB (269 words) - 09:44, 11 September 2020
- ...n of bovine serum albumin and to the choice of chelating agent used in the preparation and assay buffers. Mammary mitochondria prepared and assayed in buffers con2 KB (326 words) - 14:21, 20 May 2015
- ...ochondrial preparations]] and is useful as a quality control of the sample preparation.2 KB (276 words) - 12:36, 25 November 2020
- ''in preparation''2 KB (296 words) - 12:04, 5 September 2022
- ''Work in progress'' by [[Gnaiger E]] 2020-02-15 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m2 KB (315 words) - 12:25, 20 February 2020
- ...wer O<sub>2</sub> limit should be defined in the software according to the preparation (imt, cells, permeabilized fibers) and according to the consequent respirat3 KB (510 words) - 11:07, 25 March 2022
- ...re keen to share our experience in human permeabilized muscle fiber bundle preparation. To this end we feel that our contribution may best benefit the scientific2 KB (318 words) - 06:48, 4 March 2020
- ...syndrome; and (3) heterozygote animals. A newly developed micro-method for preparation and assaying of small quantities of intact mitochondria was employed. With2 KB (304 words) - 17:56, 16 January 2021
- ...e pooling brain tissue from six to nine animals for a single mitochondrial preparation. Working with pooled tissue significantly decreases the quality of data bec2 KB (311 words) - 17:51, 22 February 2021
- ...edures are required regarding instrumental settings, sample collection and preparation, protocol design and respirometric data analysis of mitochondrial respirato2 KB (311 words) - 14:40, 5 July 2023
- ...f mitochondrial function in saponin-permeabilized cardiomyocyte bundles, a preparation where all mitochondria are represented and their structure intact, from you2 KB (309 words) - 09:47, 8 November 2016
- ...ility of skeletal muscle to respire lactate using an in situ mitochondrial preparation that leaves the native tubular reticulum and subcellular interactions of th2 KB (326 words) - 12:05, 28 March 2018
- ...y the approach to discover that inclusion of phosphatase inhibitors in the preparation of the heart mitochondria results in a specific decrease in rates of Comple2 KB (344 words) - 10:48, 26 February 2015
- ...ygen consumption rate. Assays typically require 3 h after overnight sample preparation. Companion methods are also discussed and provided to aid researchers in de2 KB (310 words) - 15:52, 4 June 2022
- :::: '''Preparation of 500 mM ATP stock solution''' :::: ''' Preparation of 500 mM ATP stock solution with 400 mM free Mg<sup>2+</sup> '''5 KB (732 words) - 15:07, 17 October 2022
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[mitObesity drugs]].2 KB (292 words) - 14:09, 24 September 2023
- ...a, permeabilized tissues, or permeabilized cells, adding the mitochondrial preparation to respiration medium containing inorganic phosphate ([[State 1]]), then ad |?Preparation=Preparation12 KB (1,658 words) - 01:03, 31 December 2020
- ...of 5 mM ATP could stimulate oxidation of octanoate or palmitate in either preparation, suggesting that these tissues have little capacity to oxidize fatty acids2 KB (328 words) - 09:11, 9 November 2016
- ...This enhancement of the antioxidant system - coined over a decade ago as "preparation for oxidative stress" - controls the possible harmful effects of increased2 KB (349 words) - 12:24, 31 May 2019
- ==== Preparation of MgG solution ==== ::: '''Preparation of 1.1 mM stock solution''' (dissolved in H<sub>2</sub>O):17 KB (2,616 words) - 15:42, 7 February 2024
- :::: Preparation of a review paper with the tentative title, βAltered function of cardiac2 KB (301 words) - 12:00, 23 October 2019
- ...ence of organs or the whole body to severe hypoxic insults is an important preparation for high-altitude sojourns or to protect the cardiovascular system from hyp3 KB (341 words) - 06:49, 28 June 2022
- |additional=Blood cell preparation2 KB (333 words) - 14:59, 13 November 2017
- ...t=The paper considers the effects of bedaquiline (BDQ), an antituberculous preparation of the new generation, on rat liver mitochondria. It was shown that 50β―ΞΌ2 KB (344 words) - 15:34, 2 January 2019
- ...sticsβ. Experts in the field meet to share their knowledge on blood sample preparation, pre-clinical analysis, functional measurements and data analysis, as well3 KB (324 words) - 10:21, 9 August 2023
- ...atory adenylate control ratios and cytochrome c conservation in the tissue preparation were comparable or even better than in isolated mitochondria. Citrate synth2 KB (327 words) - 14:05, 27 December 2021
- ...t=The paper considers the effects of bedaquiline (BDQ), an antituberculous preparation of the new generation, on rat liver mitochondria. It was shown that 50β―ΞΌ2 KB (347 words) - 14:03, 2 July 2019
- ...s of oxidative phosphorylation (OXPHOS) in saponin-skinned cardiac fibers. Preparation of permeabilized myocardial fibers allows the assessment of respiratory fun2 KB (325 words) - 11:42, 23 January 2019
- ...ity is impaired in the pancreatic tissue of rats with experimental AP. For preparation of mitochondria from rat pancreas, routine isolation procedures (tissue hom2 KB (350 words) - 11:03, 27 March 2018
- ...s vinblastine had no effect. Utilizing a permeabilized muscle fiber bundle preparation that retains the cytoskeleton, paclitaxel treatment impaired the ability of3 KB (336 words) - 13:47, 16 August 2019
- ...ure and so decrease the loss of [3H]DOG from the mitochondria during their preparation.3 KB (369 words) - 08:36, 16 June 2015
- Methods: C6 cell culture was used for experiments. Cell breeding, preparation, density calculation was executed by the methods of cell cultivation. Mitoc3 KB (369 words) - 16:15, 14 February 2018
- ...proaches can be applied to tissue homogenates, which simplifies the sample preparation and reduces the required starting material compared with isolating mitochon2 KB (335 words) - 13:42, 21 July 2023
- ...rm collaboration with Martin's team to study and train the Alaskan dogs in preparation of long-distance races, the most challenging of which is the 1,100 mile Idi File:Robert.jpg|Robert Boushel at muscle fibre preparation with the new Oroboros cooling plate. The snow for cooling BIOPS and MiR06Cr12 KB (1,768 words) - 02:24, 9 October 2019
- ::::'''Preparation of 250 mM stock solution (dissolved in DMSO)''':2 KB (337 words) - 22:29, 9 April 2022
- ::::# Completed SOPs for cell preparation & laboratory protocols.3 KB (390 words) - 16:47, 12 January 2018
- ...buffer system in the revised medium. Other advantages include more facile preparation of trace element stock solutions, a reduction in total chemical use, a more2 KB (354 words) - 15:15, 8 August 2023
- ...drial fitness score. To this end, SOPs will be worked out regarding sample preparation, respiratory evaluation and data documentation. Fresh and cryopreserved cel3 KB (357 words) - 18:40, 10 January 2022
- ...drial fraction of rat liver by isopycnic centrifugation. The purity of our preparation was verified by estimation of the respective marker enzymes. Analysis of ly3 KB (388 words) - 22:18, 16 February 2021
- ...and lung functions were evaluated respectively by means of isolated heart preparation, bronchoalveolar lavage fluid protein concentration, lung wet/dry weight ra3 KB (360 words) - 11:50, 22 December 2020
- ...OXPHOS. The major source of variation in OXPHOS measurements is the sample preparation. The thigh involved, time-point of collection, size of fiber bundles, and t3 KB (369 words) - 17:13, 7 March 2020
- File:MiPArt4_16.JPG|The new OROBOROS sculpture by Harald Kirchebner, in preparation for the barbecue of the chestnuts.3 KB (485 words) - 15:56, 4 May 2017
- ...d data together. Mainly due to difference in the protocols used for sample preparation, different animal models and strains, different SUIT protocols - including3 KB (388 words) - 15:55, 27 March 2018
- :::: '''Preparation of 1 mM stock solution''' (dissolved in Ethanol) for '''2-mL O2k-chamber''' :::: '''Preparation of 0.25 mM stock solution''' (dissolved in Ethanol) for '''0.5-mL O2k-chamb6 KB (845 words) - 18:22, 12 September 2022
- ...ally different skeletal muscles is compared. Data for a single ectothermic preparation are also reported. The mitochondria were prepared in yields of 44+/-7% from2 KB (336 words) - 17:57, 16 January 2021
- :::: '''Preparation of 2 M stock solution''' (dissolved in H<sub>2</sub>O)3 KB (352 words) - 09:26, 4 July 2023
- |additional=Blood cell preparation3 KB (362 words) - 17:21, 4 June 2017
- ...''M. oleifera'' seeds to treat water, since this lectin is present in the preparation employed by some populations to this end.3 KB (370 words) - 21:33, 20 August 2020
- ...ion of infarct sizes in isolated perfused hearts. We therefore developed a preparation of cardiomyocytes from a single mouse heart, where aliquots of the same hea3 KB (352 words) - 15:31, 28 November 2022
- ...tion. We also found that the addition of DNAse altered Var1 content in the preparation of mitochondrial nucleoids, which is indicative of binding. Importantly, Va3 KB (365 words) - 11:23, 28 April 2017
- ...or re-oxygenations to extend measurement of oxygen flux. The mitochondrial preparation must be stable over such experimental periods of time, hence specific mitoc3 KB (339 words) - 08:57, 27 May 2020
- ...onitoring of a mitochondrial fitness score. SOPs are elaborated for sample preparation, respiratory evaluation and data documentation. Fresh and cryopreserved cel3 KB (342 words) - 18:53, 10 January 2022
- ...Inter-laboratory study on human permeabilized myofibers: effects of fiber preparation, respiration buffer, and blebbistatin on mitochondrial respiratory capacity ...(HRR) comparing in parallel (n=16 pfi) the following conditions: (1) fiber preparation; (2) respiration media MiR05-Kit and Buffer Z in the presence of the myosin7 KB (952 words) - 19:05, 10 January 2022
- ...ptly falls by a factor of 4- to 10-fold, depending upon the quality of the preparation. This abrupt decrease of respiration is due to the high affinity of the oxi3 KB (391 words) - 13:31, 29 May 2022
- |?Preparation=Preparation |?Preparation=Preparation11 KB (1,488 words) - 20:30, 19 March 2022
- ...asons, we have decided to go ahead with our presented project plan and the preparation of the proposal for '''Horizon2020 SME Instrument Phase 2'''.3 KB (432 words) - 09:32, 4 April 2022
- ...rometry based assay vs radiolabeled substrate based technique) and type of preparation (isolated mitochondria vs cell lines). :::: β’ Comparison of instrumental platforms, assays, and type of preparation6 KB (904 words) - 12:30, 17 March 2020
- in preparation4 KB (466 words) - 10:56, 11 April 2022
- :::: '''Preparation of 400 Β΅M stock solution''' (dissolved in ethanol abs.)3 KB (370 words) - 11:04, 14 June 2022
- ...xygen concentration. This technique requires < 20 mg of myocardium and the preparation of the sample takes <20 min. Mitochondria in the homogenate, as compared to3 KB (373 words) - 03:31, 18 April 2024
- :::: Presently an innovation is in preparation and will be available as a new product: a stainless steel mesh is used to m3 KB (425 words) - 16:12, 1 March 2021
- ...yme was purified 100-fold from calf adrenal cortex mitochondria. The final preparation was free of malic dehydrogenase, fumarase, the strictly NADP+-linked malic3 KB (443 words) - 13:50, 27 February 2015
- ...easing ''FCF<sub>c</sub>'' (Figure 1). Apparently, the damage caused by mt-preparation even in cases with ''FCF<sub>c</sub>'' up to 0.25 could be rescued by addit # Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with ref6 KB (853 words) - 18:30, 10 January 2022
- ...ATPase activity in [[isolated mitochondria]] suggests contamination of the preparation by non-mitochondrial membranes. Endogenous adenylates are phosphorylated to3 KB (400 words) - 12:36, 25 November 2020
- were obtained for the subsequent determinations. Pharmaceutical preparation of melatonin in gel is currently under patent.3 KB (409 words) - 00:58, 10 February 2020
- ...art failure patients obtained during open heart surgery by standard tissue preparation or ''ex vivo'' endomyocardial biopsy (rβ=β0.9988, pβ<β0.0001, nβ=3 KB (387 words) - 13:21, 7 March 2020
- ::: '''Preparation of the 5 mM stock solution of DTPA:'''3 KB (417 words) - 15:55, 4 April 2024
- ...l to exclude potential injuries caused experimentally during mitochondrial preparation by tissue homogenization or plasma membrane permeabilization.3 KB (413 words) - 14:32, 9 May 2023
- ...s depend on equal distribution of subsamples obtained from a homogenous mt-preparation or determination of a common [[mitochondrial marker]].3 KB (427 words) - 10:40, 24 October 2023
- ...is were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mass spectrometry. "Total Pr3 KB (405 words) - 18:17, 6 November 2020
- ...suggests that image duplication results from systematic problems in figure preparation by individual researchers, which tend to recur.3 KB (492 words) - 20:13, 3 December 2023
- ...iption='''Permeabilized muscle fibers''' (pfi) are used as a mitochondrial preparation in respirometry to access mitochondrial function comparable to [[isolated m ...a physiological temperature of 37 Β°C, therefore, it must be shown that the preparation is not oxygen limited due to the spatial configuration of fiber bundles and23 KB (3,156 words) - 08:57, 18 May 2022
- ...line and with lipopolysaccharide (LPS). Fresh PBMC isolated using the cell preparation tube generated optimum mitochondrial responses. Intact cell and maximal res3 KB (435 words) - 12:20, 12 July 2023
- :::: '''Preparation of 100 mM stock solution''' (dissolved in H<sub>2</sub>O or DMSO, can diffe3 KB (430 words) - 10:59, 4 April 2024
- ...|Inter-laboratory study on human permeabilized myofibres: effects of fibre preparation, respiration buffer, and blebbistatin on mitochondrial respiratory capacity3 KB (429 words) - 14:40, 13 November 2019
- ...es were collected from a Robinia pseudoacacia tree in the fall. The sample preparation involved the cutting and homogenization of leaves in two different media. F4 KB (491 words) - 21:13, 30 April 2024
- ::::* For preparation of MiR05, see [[MiPNet22.10 MiR05-Kit]].3 KB (410 words) - 16:13, 14 December 2023
- ''in preparation''3 KB (443 words) - 14:38, 18 December 2023
- ...unning instrument. Afterwards, an adequate design of the protocols (sample preparation, SUIT protocols) are needed to obtain optimal results. In the context of Mi3 KB (448 words) - 14:49, 21 December 2021
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m3 KB (464 words) - 22:50, 8 March 2020
- ...potential, ADP-ATP exchange, Ca<sup>2+</sup> dynamics). Any mitochondrial preparation (mtprep) can be applied, from isolated mitochondria, tissue homogenate, per3 KB (440 words) - 14:23, 5 July 2023
- ...wer O<sub>2</sub> limit should be defined in the software according to the preparation (imt, cells, permeabilized fibers) and according to the consequent respirat5 KB (737 words) - 13:05, 24 March 2022
- ...siness relations with any organization involved in this research or in the preparation of the manuscript?5 KB (689 words) - 15:14, 22 November 2022
- ...bundles were kept on ice in the same solution until respirometry analysis. Preparation of permeabilized βghostβ muscle fibers: Fibers were [...] washed three8 KB (1,122 words) - 11:21, 23 December 2022
- :::: '''Preparation of 1 M stock solution:'''3 KB (467 words) - 15:06, 17 December 2022
- ...rmally do not present mitochondrial outer membrane damage after the sample preparation, e.g. human permeabilized muscle fibers.3 KB (496 words) - 12:08, 17 September 2020
- ...temperatures (4, 25, 30, 37 and 40 Β°C) for each mouse left ventricle fiber preparation (NΒ³4). At 37 Β°C, the respiratory control ratio (malate+pyruvate; stimulat3 KB (453 words) - 18:14, 10 January 2022
- ...gh-resolution respirometry with a measurement regimen optimized for tissue preparation and substrate supply. Evaluation of mitochondrial function in skin biopsies3 KB (499 words) - 08:43, 18 March 2020
- :::: Preparation of joint manuscript for publication. Templates for PBMC and PLTdatabase Wor3 KB (438 words) - 15:36, 23 November 2021
- ...x.php/File:Lemieux_et_al_v-Raf-2019-05-28.pdf '''Version 2 (2019-06-11) in preparation'''], [http://biorxiv.org/content/early/2017/06/19/151480 '''bioRxiv Preprin3 KB (477 words) - 11:08, 27 November 2021
- File:Greenland3.jpg|SΓΈren in good spirit during preparation for the arm biopsy. ...three Oroboros O2k, to analyze replicate samples of muscle biopsies after preparation of permeabilized fibres (CMRC, Rigshospitalet, Copenhagen; February 2004).8 KB (1,191 words) - 15:21, 1 December 2019
- ...t-workshop at the beginning of the MSC, involving all participants); fibre preparation; comparison with isolated mitochondria and homogenate; statistical analysis3 KB (493 words) - 04:00, 19 November 2019
- | Manuscript preparation: Results4 KB (498 words) - 15:36, 23 November 2021
- ...drial fitness score. To this end, SOPs will be worked out regarding sample preparation, respiratory evaluation and data documentation. Fresh and cryopreserved cel3 KB (464 words) - 15:43, 14 February 2019
- ...rmally do not present mitochondrial outer membrane damage after the sample preparation, e.g. human permeabilized muscle fibers.4 KB (543 words) - 15:19, 8 June 2020
- # Masola B, Evered DF (1984) Preparation of rat enterocyte mitochondria. Biochem J 218: 441-7.3 KB (471 words) - 12:44, 23 January 2019
- ...IT protocol in use in host lab with results in soleus to compare the fiber preparation. Then I will perform HRR experiments with the protocol for permeabilized fi3 KB (513 words) - 15:28, 16 March 2020
- ...o analyse the mitochondria tissue homogenization with Shredder PSI-HRR for preparation of sample was used and tested against Biops with/Saponin, which dissolves c4 KB (564 words) - 14:33, 13 November 2017
- ...ory control: Building blocks of mitochondrial physiology]] - Manuscript in preparation by the MitoEAGLE Terminology Group.4 KB (512 words) - 11:57, 23 October 2019
- ...e activities). Respiratory capacity in a defined respiratory state of a mt-preparation can be considered as a functional mt-marker, in which case respiration in o4 KB (536 words) - 13:43, 26 February 2021
- The mitochondrial preparation from non-recruited ingWAT was qualitatively different from IBAT mitochondri4 KB (515 words) - 16:01, 13 November 2017
- ...led as described in [3]. Isolation of mitochondria from cultured cells and preparation of cell homogenates, mitochondrial membrane-enriched and cell soluble fract4 KB (573 words) - 11:23, 13 April 2015
- ...emical permeabilization agents or sample processing constitutes a reliable preparation method for OXPHOS analysis in small amounts of prostatic tissue typically o4 KB (509 words) - 04:36, 19 July 2022
- ...mple was stimulated by the addition of succinate with rotenone and ADP and preparation was left to consume all O<sub>2</sub> in respiratory chamber (within 10-204 KB (521 words) - 12:48, 17 March 2019
- ...tics]]. Experts in the field meet to share their knowledge on blood sample preparation, pre-clinical analysis, functional measurements and data analysis, as well4 KB (548 words) - 10:50, 24 October 2023
- ...some chemicals the entire commercial stock may have to be used at once for preparation of a storage solution or stock solution.5 KB (791 words) - 02:57, 23 January 2023
- ...isolated mitochondria would require minimal amount of tissue and time for preparation and therefore could be widely used for diagnostic purposes.4 KB (522 words) - 18:26, 10 January 2022
- a mitochondrial preparation at 540nm can follow mitochondrial swelling after MPT pore4 KB (488 words) - 12:56, 27 June 2019
- :::'''Preparation of 5 mM storage solution (dissolved in DMSO)''' :::'''Preparation of 1 mM stock solution'''17 KB (2,405 words) - 07:29, 5 April 2024
- ...ientific report and of short paper (or new data elaboration) March 19-23rd Preparation of scientific report and short paper (or and a new presentation to the host11 KB (1,641 words) - 11:22, 6 July 2022
- ...detergents ([[digitonin]]), to obtain the [[Mitochondrial preparations |mt-preparation]] of [[permeabilized cells]] used for [[cell ergometry]]. Living cells are |mitopedia topic=Sample preparation9 KB (1,309 words) - 10:48, 12 October 2022
- :::: ''in preparation''5 KB (573 words) - 09:44, 29 June 2023
- :::# Training of researchers towards improved reproducibility of sample preparation and respirometric evaluation :::# A library of protocols for sample preparation and examination by respirometry11 KB (1,507 words) - 12:05, 19 January 2018
- ...en Access at Zenodo repository: https://doi.org/10.5281/zenodo.6323145 (in preparation)5 KB (706 words) - 11:19, 3 April 2024
- ...r, from the clinicianβs request and including the examination requisition, preparation of the patient, collection of the primary sample, and transportation to and5 KB (743 words) - 05:12, 19 July 2022
- ...Fig. 1. Workflow in the pre-analytical and analytical phases of blood cell preparation and respiration in living cells (ce) and permeabilized cells (pce).]] ...resenting and discussing the respirometric results obtained with different preparation methods.15 KB (1,990 words) - 14:39, 5 July 2023
- ...s of kinases and ATPases might be necessary depending on the mitochondrial preparation used, specially for permeabilized cells or tissue homogenates ([[Chinopoulo5 KB (755 words) - 10:43, 18 August 2023
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m5 KB (721 words) - 07:53, 7 March 2024
- ...due to application of different experimental procedures, including sample preparation, substrate-uncoupler-inhibitor titrations (SUIT), respiratory media, and ox ...hamber. Protocols were compared at several levels: (1) permeabilized fiber preparation; (2) respiration media MiR05-Kit and Buffer Z in the presence/absence of bl9 KB (1,242 words) - 08:27, 8 January 2023
- ::::#Draxl A, Eigentler A, and Gnaiger E (2013) PBI-Shredder HRR- Set: preparation of tissue homogenates for diagnosis of mitochondrial respiratory function.4 KB (587 words) - 16:11, 12 January 2018
- ...s of kinases and ATPases might be necessary depending on the mitochondrial preparation used, especially for permeabilized cells or tissue homogenates ([[Chinopoul5 KB (762 words) - 11:12, 18 August 2023
- ...ntly found by Green et al. (7) that tryptic digestion of their particulate preparation, the βsuccinic dehydrogenase complex,β liberated appreciable amounts of5 KB (718 words) - 20:57, 31 March 2023
- ''Work in progress'' by [[Gnaiger E]] 2020-01-20 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m5 KB (707 words) - 22:55, 8 March 2020
- ...will also discuss some methodological consideration with regards to sample preparation.4 KB (556 words) - 23:56, 17 March 2019
- ...]]'''PBI-Shredder O2k-Set''': Auxiliary O2k-Tool for [[tissue homogenate]] preparation5 KB (653 words) - 11:05, 5 August 2022
- ...> depend on available substrates and the prevailing coupling state of a mt preparation [2]. Details of the interrelationship between respiratory states and Ξ''Ξ¨5 KB (717 words) - 15:43, 14 February 2019
- ...effect shows the integrity of the outer mitochondrial membrane after fibre preparation and also shows the preservation of integrity over a 90-100 min incubation i5 KB (721 words) - 15:01, 8 June 2020
- ::: '''Preparation of 1 mM stock solution''' ...RM]] or [[Rhodamine 123]] to mtMP [mV] requires a specific method for each preparation and protein content, which has to be kept constant in an experimental serie13 KB (1,842 words) - 17:09, 17 January 2024
- :::: Moreover, several projects are still in the process of final manuscript preparation, such as harmonization of protocols, which not only help to create clear gu ...ation of protocols across research groups and to compare protocols (sample preparation, experimental details)29 KB (4,066 words) - 11:09, 16 March 2021
- ...chondrial phosphate/oxygen ratio by measuring ATP levels fluorometrically. Preparation and execution of each method will be learned with emphasis on internal qual5 KB (655 words) - 09:51, 23 February 2024
- ...ty to activation upon blood collection, as well as in the course of sample preparation, largely contributes to possible artifactual observations encountered when5 KB (684 words) - 16:30, 13 November 2017
- ...preference may be to carry out an experiment with cells or a mitochondrial preparation used in the lab.6 KB (801 words) - 23:59, 17 March 2019
- ...e of variation in measuring mitochondrial oxidative capacity is the sample preparation per se were shown. Furthermore, other factors such as the possible differen6 KB (840 words) - 17:16, 7 March 2020
- ...ify the sources of variability found in the MitoEAGLE WG2 pilot study. The preparation of the samples will be performed according the SOP established for the Mito6 KB (675 words) - 14:21, 20 October 2022
- ::::::: Further reading: Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria of integrity, and assays with ref6 KB (897 words) - 20:07, 22 February 2022
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m6 KB (966 words) - 11:53, 8 March 2020
- Pic. 1: sample preparation in our "field laboratory"5 KB (715 words) - 09:41, 3 March 2020
- ...|Inter-laboratory study on human permeabilized myofibres: effects of fibre preparation, respiration buffer, and blebbistatin on mitochondrial respiratory capacity ...GLE blood cells 1]] - A data manager will collect and analyze all data, in preparation of a database for Open Access in conjunction with the journal publication14 KB (1,939 words) - 15:46, 6 November 2019
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m5 KB (831 words) - 22:51, 8 March 2020
- ...orated for evaluation of ''Ξ¨<sub>mt</sub>'' for each type of mitochondrial preparation and protein concentration [3] that has to be kept constant for comparison o6 KB (883 words) - 18:36, 10 January 2022
- ...oros system is unique in allowing this type of studies using intact tissue preparation, making it possible to evaluate mitochondrial function in structures for wh6 KB (863 words) - 18:13, 16 March 2020
- ...echanical and chemical permeabilization as the preferred choice for tissue preparation, since it offers critical advantages over commonly used alternatives (i.e.,6 KB (821 words) - 08:35, 28 July 2022
- ...e and sample requirement while preserving the quality of the mitochondrial preparation.33 KB (4,250 words) - 15:07, 2 February 2022
- ''Work in progress'' by [[Gnaiger E]] 2020-02-14 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m6 KB (867 words) - 00:35, 15 February 2020
- ::: [[Property:Preparation]]7 KB (696 words) - 11:14, 20 October 2017
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[mitObesity drugs]].6 KB (852 words) - 15:51, 20 November 2023
- ...Power-O2k approach (6 instruments, 12 chambers) and fast tissue-homogenate preparation with the PBI-Shredder will provide the basis for a statistically complete s6 KB (865 words) - 09:23, 19 February 2020
- File:IOC104 4.JPG| Peter showing a video of the fiber preparation.6 KB (999 words) - 15:14, 8 June 2020
- ...ell culture techniques, small needle biopsies of muscle, and mitochondrial preparation methods. Multiple substrate-uncoupler-inhibitor titration (SUIT) protocols6 KB (850 words) - 12:27, 5 March 2024
- ''Work in progress'' by [[Gnaiger E]] 2020-02-09 linked to a preprint in preparation on [[mitObesity drugs]].6 KB (850 words) - 14:08, 24 September 2023
- ...s, we suggest testing the effect of 50 mM S on these rates in each type of preparation before using it routinely in the SUIT protocol.7 KB (1,014 words) - 19:16, 10 January 2022
- :* Preparation of protocols and recommendations.7 KB (1,008 words) - 10:21, 23 January 2019
- ...lating poorly with cristae density. The saponin-permeabilized muscle fibre preparation eliminates the need for a time-consuming and disruptive isolation procedure8 KB (1,107 words) - 12:10, 27 March 2018
- .../d/193Mhl-2p4Q_sgIEhE9tGmynthOxOUjuu2zqf2sWxrwk/edit#gid=0 here] for an in preparation database of preprint commentary venues).8 KB (1,155 words) - 19:55, 17 January 2021
- Abstract in preparation:6 KB (912 words) - 18:51, 10 January 2022
- | ''' Is your mitochondrial preparation contaminated with other membranous organelles containing ATPases?''' <br> ...through the SUIT-024 as a quality control protocol of the [[mitochondrial preparation]] <br>21 KB (2,889 words) - 13:31, 19 January 2021
- ...ilized which, as previously reported, represents a very good mitochondrial preparation alternative to isolated mitochondria, both reducing the amount of required7 KB (962 words) - 18:31, 10 January 2022
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[body mass excess |'''BME''']] and [[:Category:BME and mitObesity |'''m7 KB (1,022 words) - 22:52, 8 March 2020
- :::: 1.Permeabilized fiber preparation: Mouse heart.9 KB (1,112 words) - 14:44, 25 January 2020
- {'''Which mitochondrial preparation technique is most suitable for studying the effects of specific drugs on AT ...is it crucial to maintain the integrity of mitochondrial membranes during preparation?'''36 KB (5,157 words) - 12:10, 12 April 2024
- :::: '''Preparation of 10 mM stock solution- Conditioning solution 1''' (dissolved in 100 mM KC :::: '''Preparation of 1 Β΅M stock solution- Conditioning solution 2''' (dissolved in 100 mM KC37 KB (5,710 words) - 15:30, 16 May 2023
- ...issues from mouse models and humans. The applied techniques covered sample preparation, lipidomics approaches and also protein and functional analyses including e9 KB (1,253 words) - 15:28, 9 July 2018
- : '''10:45-12:00 Workshop: Blood cell preparation, cryopreservation, respiration''' ...respiration in permeabilized fibres: respiration media, inhibitors, sample preparation'''34 KB (4,472 words) - 11:55, 12 March 2024
- ...e never seen such a professionality and group spirit during the manuscript preparation and I like it a lot. Those moments were very fascinating and completely abs ...ory control: Building blocks of mitochondrial physiology]] - Manuscript in preparation by the MitoEAGLE Terminology Group.20 KB (2,682 words) - 10:37, 28 October 2022
- ...tory protocol. Moreover, mt-respiration values are highly dependent on pfi preparation, which required good technical skills.7 KB (971 words) - 12:55, 21 February 2023
- ...tory protocol. Moreover, mt-respiration values are highly dependent on pfi preparation, which required good technical skills.8 KB (1,064 words) - 08:25, 8 January 2023
- ...trations (how to evaluate correct or optimal concentration; stock solution preparation)9 KB (1,240 words) - 22:45, 14 November 2022
- An important step in the introduction of a new antibacterial preparation into medical practice is the study of its influence on the eukaryotic cell,8 KB (1,282 words) - 12:03, 22 September 2018
- ...)). The apparent difference is not a property of the type of mitochondrial preparation. In general, thresholds and spare capacities of OXPHOS components downstrea8 KB (1,121 words) - 19:16, 10 January 2022
- In preparation - last update 2023-07-2010 KB (1,476 words) - 08:43, 24 July 2023
- ::::# External energy substrates have to be added to the preparation, since the endogenous substrates of the cytoplasm have been removed. A resi12 KB (1,557 words) - 20:27, 19 March 2022
- ...ents, the 'DataSchema' and 'Harmonization Platforms', together support the preparation of effective data-collection protocols and provide a central reference to f11 KB (1,593 words) - 15:14, 10 December 2015
- ...: [[Markova 2016 Abstract MitoFit Science Camp 2016|Porcine hearts samples preparation: PBI-Shredder or saponin?]] :::: '''B1''': Mitochondrial assays in blood cells: [[Blood cell preparation]], cryopreservation, respiration.27 KB (3,721 words) - 13:27, 3 April 2022
- ...able in the lab (e.g. [[ISS]]), or little aids that are crucial for tissue preparation (forceps), mitochondrial respiration medium ([[MiR05-Kit]]), [[O2k-Procedur14 KB (1,888 words) - 18:25, 29 March 2024
- ...linical practice. The main concerns and discrepancies were observed in the preparation of the sample, specifically the isolation procedure used to obtain quality11 KB (1,482 words) - 12:29, 8 May 2024
- ...armonized reference protocols 1 and 2 (SUIT-001 and 002) for mitochondrial preparation.12 KB (1,521 words) - 14:23, 15 May 2023
- [[File:ORO Sitzkreis.JPG|250px|Preparation]] [[File:Hands-on Session 5.JPG|250px|Hands-on Session]] [[File:Hands-on Se12 KB (1,700 words) - 15:12, 8 June 2020
- A manuscript in preparation for publication on βMitochondrial states and ratesβ is the first prepri ...of their possible relevance for my research or the manuscript currently in preparation for submission? Is another βpaperβ added to the masses still considered28 KB (3,945 words) - 08:24, 8 January 2023
- ...inued '''TC'''-mode of the Unrealist provides escape. I have a living cell preparation on the bench, but all are dying, without application of any mild detergent17 KB (2,764 words) - 11:11, 24 December 2021
- Permeabilized fiber preparation: Mouse heart.12 KB (1,502 words) - 15:07, 22 March 2017
- File:IOC103 0411.JPG|Preparation of chemicals.13 KB (1,875 words) - 00:16, 12 January 2022
- :::: '''Preparation of 10 mM stock solution''' (dissolved in DMSO) - see also [http://tools.lif :::: Note: The preparation of the 10 mM storage solution follows the procedure suggested by the manufa35 KB (5,265 words) - 15:10, 25 April 2024
- | Preparation of single cell suspensions / isolated mitochondria from cultured cells / mu ...Os). We have been using earlier rat and trout cardiomyocytes as well. As a preparation we use either intact cells or saponin-permiabilized cells. From the techniq41 KB (5,420 words) - 15:06, 23 November 2020
- ...ial preparation protocols, including biopsy sampling, tissue storage, cell preparation protocols, etc. ...ievable at the present level of research in mt-physiology, where different preparation protocols, incubation media, and respiratory protocols are used. Therefore,56 KB (7,818 words) - 11:01, 7 April 2021
- ...espirometry (Pesta and Gnaiger 2012), is recommended for reading as a good preparation for the workshop. In addition to the literature, a random or targeted walk15 KB (2,242 words) - 14:11, 21 July 2020
- # Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with re # Tyler DD, Gonze J (1967) The preparation of heart mitochondria from laboratory animals. In: Estabrook RW, Pullman ME25 KB (3,492 words) - 06:44, 13 January 2021
- ...M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]Β«27 KB (4,026 words) - 11:53, 29 March 2021
- ''Work in progress'' by [[Gnaiger E]] 2020-02-10 linked to a preprint in preparation on [[mitObesity drugs]].15 KB (2,166 words) - 14:12, 24 September 2023
- :::: Following the preparation of necessary solutions as described in [[MiPNet14.06_Instrumental_O2_backgr26 KB (3,995 words) - 10:15, 7 October 2021
- :::# Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with re17 KB (2,349 words) - 21:17, 14 May 2024
- ...Bharadwaj MS, Tyrrell DJ, Lyles MF, Demons JL, Rogers GW, Molina AJ (2015) Preparation and respirometric assessment of mitochondria isolated from skeletal muscle ...permeabilized muscle fibers, isolation of mitochondria does require longer preparation time. Also, loss of cellular content diminishes physiological relevance, so52 KB (7,111 words) - 14:50, 13 January 2023
- :::# While biopsy sampling and fiber preparation proceed: Perform air calibration in [[MiR06Cr]], then close the chamber to22 KB (3,238 words) - 15:29, 2 May 2024
- :::# Liakh, I. et al. (2019) βModern Methods of Sample Preparation for the Analysis of Oxylipins in Biological Samples.β, Molecules (Basel,17 KB (2,106 words) - 08:18, 8 January 2023
- ...ial respirometry will be developed, from standardized protocols and sample preparation to the world-wide innovation of a proficiency ring test. These developments ...ocols including instrumental performance protocols, mitochondrial and cell preparation protocols and experimental titration protocols (SUIT protocols; Fig. 2).43 KB (5,966 words) - 07:05, 29 March 2024
- ...uosos", in the 20th century meaning of the word. (An English edition is in preparation.)21 KB (3,259 words) - 09:04, 12 December 2017
- ...tory of up-to-date reporting guidelines and other resources assisting in a preparation of good research manuscript (www.equator-network.org).29 KB (4,069 words) - 08:22, 22 May 2024
- |''' "In respirometric studies on blood cells, the purity of cell preparation should always be emphasized and documented." '''<br>31 KB (4,505 words) - 09:37, 21 May 2024
- # Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with re34 KB (4,820 words) - 04:08, 23 November 2021
- :::# Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with re :::# Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with re89 KB (12,074 words) - 17:54, 6 May 2024
- ...M8v0&feature=youtu.be O2k-Videosupport: PBI-Shredder SG3 tissue homogenate preparation]Β« | ''' You can quantify the ATPase activity in your mitochondrial preparation:'''126 KB (17,785 words) - 08:55, 13 August 2021
- :::# Rasmussen HN, Rasmussen UF (1997) Small scale preparation of skeletal muscle mitochondria, criteria for integrity, and assays with re51 KB (7,005 words) - 04:36, 19 July 2022
- ...ass Excess, mitObesity, and mitochondrial fitness. MitoFit Preprints. ''in preparation'' v0. ...is not even covered in the ''X''-mass carol you heard last night. It is in preparation for next year's version, as discussed in many COSTly events already. || COS171 KB (27,973 words) - 19:50, 27 December 2021
- |'''Improve preparation of permeabilized muscle fibers'''85 KB (11,819 words) - 14:18, 10 January 2022
- ...amage of the mitochondrial outer membrane, which could be caused by sample preparation or pathological conditions. <br>80 KB (11,165 words) - 09:37, 8 January 2024
